Nanozyme-based cascade reaction has emerged as an effective strategy for disease treatment because of its high efficiency and low side effects. Herein, a new and highly active two-dimensional Pd-Ru nanozyme is prepared and then integrated with uricase and red blood cell (RBC) membrane to fabricate a tandem nanoreactor, Pd-Ru/Uricase@RBC, for hyperuricemia treatment. The designed Pd-Ru/Uricase@RBC nanoreactor displayed not only good stability against extreme pH, temperature and proteolytic degradation, but also long circulation half-life and excellent safety. The nanoreactor can effectively degrade UA by uricase to allantoin and H₂O₂ and remove H₂O₂ by using Pd-Ru nanosheets (NSs) with the catalase (CAT)-like activity. More importantly, the finally produced O₂ from H₂O₂ decomposition can in turn facilitate the catalytic oxidation of UA, as the degradation of UA is an O₂ consumption process. By integrating the high-efficiency enzymatic activity, long circulation capability, and good biocompatibility, the designed Pd-Ru/Uricase@RBC can effectively and safely treat hyperuricemia without side effects. The study affords a new alternative for the exploration of clinical treatment of hyperuricemia.

中文翻译：

---

一种集成二维 Pd-Ru 纳米酶、尿酸酶和红细胞膜的新型级联纳米反应器，用于高效治疗高尿酸血症

基于纳米酶的级联反应因其高效和低副作用而成为一种有效的疾病治疗策略。在此，我们制备了一种新型的高活性二维 Pd-Ru 纳米酶，然后与尿酸酶和红细胞 (RBC) 膜结合，制成串联纳米反应器 Pd-Ru/Uricase@RBC，用于治疗高尿酸血症。所设计的 Pd-Ru/Uricase@RBC 纳米反应器不仅对极端 pH、温度和蛋白水解降解具有良好的稳定性，而且循环半衰期长，安全性极佳。该纳米反应器可通过尿酸酶有效降解UA为尿囊素和H ₂ O ₂并去除H ₂ O ₂通过使用具有过氧化氢酶 (CAT) 样活性的 Pd-Ru 纳米片 (NSs)。更重要的是，H ₂ O ₂分解最终产生的O ₂反过来又可以促进UA 的催化氧化，因为UA 的降解是一个O ₂消耗过程。通过整合高效酶活性、长循环能力和良好的生物相容性，设计的 Pd-Ru/Uricase@RBC 可以有效、安全地治疗高尿酸血症，无副作用。该研究为探索高尿酸血症的临床治疗提供了一种新的选择。