Herein, this work aims to reveal the gamma irradiation-assisted the sol–gel method for the synthesis of silver (Ag) modified-nickel molybdate nanoparticles (NiMoO₄; NMO NPs) and tested for their antimicrobial and antibiofilm activities against some pathogenic bacteria and unicellular fungi. The prepared samples were characterized via XRD, HR-TEM, SEM, EDX, and elemental mapping analysis. The antimicrobial potential was tested as ZOI and MIC, while antibiofilm was estimated by tube method. The detected diffraction peaks of bare NMO NPs affirmed the successful synthesis of NMO NPs without any foreign phases. Also, three diffraction peaks were detected affirming the formation of Ag NPs on the surface of NMO NPs. The average crystallite size for the bare NMO NPs and Ag@NMO NPs was found to be 71.8 nm and 48.28 nm, respectively. Also, the SEM images have illustrated the decoration of Ag NPs on the NMO surface. Further, the TEM image illustrated that the particles of NMO possess a hexagonal shape in the nanoscale regime. Also, the elemental mapping images confirm the uniform distribution of these elements over the Ag@NMO sample. Antimicrobial results revealed that the synthesized Ag@NMO NPs recorded the most significant inhibition zone more than NMO NPs against Enterococcus columbae (33.3 ± 0.115 mm), and Candida albicans (30.8 ± 0.572 mm), and the lowest MIC (0.048 µg/ml) against E. columbae. Antibiofilm activity of Ag@NMO NPs recording 94.32% for E. columbae, 91.99% for S. vitulinus, and 90.98% for C. albicans. SEM imaging in the lack of Ag@NMO NPs exhibited normally grown bacterial cells with standard typical semi-formed biofilm. After Ag@NMO NPs treatment, remarkable morphological changes; including the total lysis of the outer surface attended by deformations with the reduction in the whole viable number.

在此，这项工作旨在揭示伽马辐射辅助的溶胶-凝胶法合成银 (Ag) 改性钼酸镍纳米粒子 (NiMoO ₄; NMO NPs）并测试了它们对某些病原菌和单细胞真菌的抗菌和抗生物膜活性。通过XRD、HR-TEM、SEM、EDX和元素映射分析对制备的样品进行表征。以 ZOI 和 MIC 测试抗微生物潜力，而通过管法估计抗生物膜。检测到的裸 NMO NPs 的衍射峰证实了 NMO NPs 的成功合成，没有任何外来相。此外，检测到三个衍射峰，确认在 NMO NPs 表面形成了 Ag NPs。发现裸 NMO NPs 和 Ag@NMO NPs 的平均微晶尺寸分别为 71.8 nm 和 48.28 nm。此外，SEM 图像还说明了 NMO 表面上 Ag NPs 的装饰。更远，TEM 图像表明 NMO 颗粒在纳米尺度范围内具有六边形形状。此外，元素映射图像证实了这些元素在 Ag@NMO 样品上的均匀分布。抗菌结果表明，合成的 Ag@NMO NPs 比 NMO NPs 记录了最显着的抑菌圈。天鸽肠球菌(33.3 ± 0.115 mm) 和白色念珠菌(30.8 ± 0.572 mm)，对天鸽大肠杆菌的最低 MIC (0.048 µg/ml) 。的Ag @ NMO的NP记录为94.32％抗生物膜活性E.鸽，为91.99％S. vitulinus，以及用于90.98％白色念珠菌。缺乏 Ag@NMO NPs 的 SEM 成像显示正常生长的细菌细胞具有标准的典型半形成生物膜。Ag@NMO NPs处理后，形态变化显着；包括由变形引起的外表面的总裂解，以及整个存活数的减少。