In the published article, Figure 2 contained an extra panel (e) that was not mentioned in the caption. This panel also appears in the article's Supporting Information as Figure S3. An incorrect version had been used in the production data. The corrected version of Figure 2 is provided here as it should have appeared in the article.
Figure 2
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Dynamic DNA origami nanostructures studied with GET. a) Sketch of the L-shaped DNA origami structure with a flexible pointer with fluorescent dye (Cy3B), and upper (26.5 nm) and lower (16.1 nm) binding strands yielding GET efficiencies of 15.7% (τup = 2.6 ns) and 59.3% (τlow = 1.3 ns). b) Representative transients for 8 (gray), 7 (blue), 6 (green), and 5 (lilac) nt binding. For 7 nt binding, the fluorescence lifetime (black) is also shown with 20 ms binning. All transients were acquired at 3 µW excitation power, except for eight nt binding (1 µW). c) Normalized correlation functions averaged over several transients and corresponding frequency distribution resulting from analyzing each transient individually. The gray dashed vertical line represents the correlation time for 8 nt extracted from concatenated transients. d) Sketch of the biosensing system with a 44 nt long tether, Cy3B, and a target recognizing unit (biotin) and target (streptavidin). e) Averaged correlation functions for photons with a long microtime (>2.5 ns) for measurements of the tether fluctuations in buffer (gray), in buffer with 30% glycerol (blue) and in buffer, incubated with streptavidin (lilac) after subtracting the fit of the correlation functions calculated from photons with a short microtime (<2.5 ns). See Supporting Information for a detailed description of the gating procedure.
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