Decellularization of biotissues reduces immunogenicity, promotes cell repopulation, and improves the functional parameters of biotissues, in particular reducing calcification. However, it is important to understand the impact of decellularization technologies on the characteristics of donor allografts in comparison with the functional characteristics of conventional cryopreserved implants. In this work, we tested and applied a new hybrid method of decellularization of donor human aorta in a multicomponent system with supercritical carbon dioxide and Tween 80. We compared the histological sections, structure, and mechanical strength properties of the hybrid decellurized aorta with those of the conventional cryopreserved and chemically treated donor aorta. We found that it is possible to completely remove cells and cellular components, preserve the histoarchitecture of the tissue using the hybrid treatment with scCO₂/Water/EtOH/Tween 80 multicomponent system, and reorganize the collagen fibrils, which is manifested in the strengthening of the tissue (Δσ=20 ± 5%) and its increased elasticity (Δε = 20 ± 3%).

生物组织的去细胞化降低免疫原性，促进细胞再生，并改善生物组织的功能参数，特别是减少钙化。然而，与传统冷冻保存植入物的功能特性相比，了解脱细胞技术对供体同种异体移植物特性的影响非常重要。在这项工作中，我们在具有超临界二氧化碳和吐温 80 的多组分系统中测试并应用了一种新的供体人主动脉脱细胞混合方法。我们比较了混合脱细胞主动脉的组织学切片、结构和机械强度特性。常规冷冻保存和化学处理的供体主动脉。我们发现完全去除细胞和细胞成分是可能的，₂ /Water/EtOH/Tween 80多组分体系，重组胶原原纤维，表现为组织强化（Δσ=20±5%），弹性增加（Δε=20±3%）。