The effectiveness of producing n-caproate from food waste without external electron donors (EDs) was investigated through batch and semi-continuous fermentation. The maximum concentration of n-caproate reached 10,226.28 mg COD/L during semi-continuous fermentation. The specificity for n-caproate was the highest at 40.19 ± 3.95 %, and the soluble COD conversion rate of n-caproate reached up to 22.50 ± 1.09 % at the end of batch fermentation. The production of n-caproate was coupled with the generation of lactate as an ED to facilitate chain elongation reactions. When lactate was used as the only substrate, n-butyrate (64.12 ± 20.11 %) markedly dominated the products, instead of n-caproate (0.63 ± 0.07 %). Microbial community analysis revealed that Caproiciproducens, Rummeliibacillus, and Clostridium_sensu_stricto_12 were the key genera related to n-caproate production. In addition to n-caproate, n-butyrate dominated the products in batch and semi-continuous fermentation with a maximum specificity of 47.59 ± 3.39 %. Clostridium_sensu_stricto_7 was committed to producing n-butyrate from lactate.

通过分批和半连续发酵研究了在没有外部电子供体 (ED) 的情况下从食物垃圾中生产正己酸的有效性。在半连续发酵过程中，正己酸的最大浓度达到了 10,226.28 mg COD/L。正己酸的特异性最高，达到40.19±3.95%，分批发酵结束时正己酸的可溶性COD转化率高达22.50±1.09%。生产N-己酸偶联与乳酸的产生作为ED以促进链延长反应。当乳酸盐被用作唯一的底物时，正丁酸盐 (64.12 ± 20.11 %) 显着地占主导地位，而不是正己酸酯 (0.63 ± 0.07 %)。微生物群落分析表明，Caproiciproducens、Rummeliibacillus和Clostridium_sensu_stricto_12是与正己酸生产相关的关键属。除了正己酸，正丁酸在分批和半连续发酵中占主导地位，最大特异性为 47.59 ± 3.39 %。Clostridium_sensu_stricto_7致力于从乳酸生产正丁酸盐。