A process engineering strategy was investigated towards developing a viable scheme for effective conversion of hydrothermolysis pretreated non-detoxified switchgrass hydrolysates (SH) to acetone butanol ethanol (ABE) using a metabolically engineered strain of Clostridium beijerinckii NCIMB 8052, C. beijerinckii_AKR. The engineered strain was modified by homologous integration into the chromosome and constitutive expression of Cbei_3974, which encodes an aldo–keto reductase. Intermittent feeding strategy was employed in which fermentation was initiated with 30% of the SH and the remaining 70% SH was added when the optical density (OD_600nm) of C. beijerinckii attained 0.5. The ABE (14.9 g/L) produced from non-detoxified SH by the inhibitor-tolerant C. beijerinckii_AKR was comparable to the P2-glucose control medium (14.7 g/L). Using intermittent feeding, wildtype and C. beijerinckii_AKR produced similar amounts of ABE (about 17.5 g/L). This shows that intermittent feeding strategy and C. beijerinckii_AKR enhanced ABE fermentation and eliminated the need for SH detoxification prior to fermentation.

使用代谢工程菌株拜氏梭菌NCIMB 8052, C. beijerinckii _AKR ，研究了一种工艺工程策略，以开发一种可行的方案，用于将水热解预处理的未解毒柳枝稷水解物 (SH) 有效转化为丙酮丁醇乙醇 (ABE) 。的工程改造的菌株通过同源整合到染色体上以及组成型表达改性Cbei _3974，其编码醛酮还原酶。采用间歇补料策略，其中以 30% 的 SH 开始发酵，并在C. beijerinckii的光密度 (OD _600nm )时加入剩余的 70% SH达到 0.5。由耐受抑制剂的拜氏念珠菌_AKR产生的未解毒 SH 产生的 ABE (14.9 g/L)与 P2-葡萄糖对照培养基 (14.7 g/L) 相当。使用间歇喂养，野生型和拜氏念珠菌 _AKR 产生相似量的 ABE（约 17.5 g/L）。这表明间歇补料策略和拜氏念珠菌_AKR 增强了 ABE 发酵并消除了发酵前对 SH 解毒的需要。