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Nuclear magnetic resonance analysis of ascorbic acid assisted lignocellulose decomposition in dilute acid pretreatment and its stimulation on enzymatic hydrolysis
Bioresource Technology ( IF 11.4 ) Pub Date : 2021-10-18 , DOI: 10.1016/j.biortech.2021.126147
Yequan Sheng 1 , Yong Xu 1
Affiliation  

The effect of ascorbic acid addition on lignin decomposition in the dilute acid pretreatment and the corresponding cellulose conversion of pretreated biomass have been studied. This enhancement by ascorbic acid addition was dose dependent. Decomposed and residual bulk lignins from pretreated poplar were analyzed by 2D HSQC and 31P NMR spectra. The promotional effect on lignocellulose decomposition with the assistance of ascorbic acid addition was supported by the NMR analysis. The analysis showed that the addition of ascorbic acid has a more significant stimulation on decomposed lignins compared to residual bulk lignins. The stimulatory effect of ascorbic acid in lignocellulose decomposition benefits the cellulose conversion of the corresponding pretreated materials. Poplar pretreatment assisted with ascorbic acid (2–8% w/w) increased the final hydrolysis yield by 7.5%–32.2%. This promotional effect of ascorbic acid on enzyme digestibility was more obvious with higher enzyme loading.



中文翻译:

稀酸预处理中抗坏血酸辅助木质纤维素分解的核磁共振分析及其对酶促水解的刺激

研究了添加抗坏血酸对稀酸预处理中木质素分解的影响以及预处理生物质的相应纤维素转化率。添加抗坏血酸的这种增强是剂量依赖性的。通过 2D HSQC 和31核磁共振谱。NMR 分析支持在添加抗坏血酸的帮助下对木质纤维素分解的促进作用。分析表明,与残留的本体木质素相比,添加抗坏血酸对分解的木质素具有更显着的刺激作用。抗坏血酸在木质纤维素分解中的刺激作用有利于相应预处理材料的纤维素转化。用抗坏血酸 (2–8% w/w) 辅助的杨树预处理使最终水解产率提高了 7.5%–32.2%。抗坏血酸对酶消化率的这种促进作用随着酶加载量的增加而更加明显。

更新日期:2021-10-20
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