This study aimed to investigate the mechanism of different treatments, namely, ultrasound (US), chlorogenic acid (CA), and ultrasound combined with chlorogenic acid (US plus CA) on the inactivation of Staphylococcus aureus planktonic and biofilm cells. Results showed that the combined treatment of US and CA exhibited remarkable synergistic antibacterial and antibiofilm effects. Scanning electron microscopy images indicated that the combined treatment of US and CA caused the most serious damage to the cell morphology. Confocal laser scanning microscopy images revealed that the combined treatment led to sharp increase and severe damage to the permeability of the cell membrane, causing the release of ATP and nucleic acids and decreasing the exopolysaccharide contents in S. aureus biofilm. Finally, the combined treatment of US plus 1% CA for 60 min inactivated S. aureus cells by 1.13 lg CFU/g on mutton. Thus, the combined treatment of US and CA had synergistic effect against S. aureus under planktonic, biofilm, and food systems.

本研究旨在探讨超声（US）、绿原酸（CA）以及超声联合绿原酸（US+CA）等不同处理对金黄色葡萄球菌浮游细胞和生物膜细胞灭活的作用机制。结果表明，US和CA联合治疗表现出显着的协同抗菌和抗生物膜作用。扫描电镜图像表明US和CA联合处理对细胞形态造成最严重的损害。共聚焦激光扫描显微镜图像显示，联合处理导致细胞膜通透性急剧增加并严重受损，导致ATP和核酸的释放，并降低金黄色葡萄球菌生物膜中的胞外多糖含量。最后，US加1%CA联合处理60分钟，使羊肉上的金黄色葡萄球菌细胞灭活1.13 lg CFU/g。因此，US和CA的联合处理在浮游、生物膜和食物系统下对金黄色葡萄球菌具有协同作用。