Rhamnolipids are glycolipid biosurfactants that have extensive applications as cleaner alternatives to chemically synthesized surfactants. The cost of producing rhamnolipids is typically high due to the poor yields realized. This cost has limited their use in commercial applications. In this study, an advanced sequential fed-batch fermentation process with high cell densities was developed for the efficient fermentative production of rhamnolipids. With this approach, the total produced rhamnolipids reached over 665 g/L by Pseudomonas aeruginosa PAO1 during the fermentation period of 18 days, with an extremely high productivity of 1.54 g/L·h. This represents a more than a 5-time improvement over the regular fed-batch fermentation. The present advanced sequential fed-batch fermentation process is extremely efficient due to the high cell density and the presence of 18 g/L NaNO₃ and 2 mg/L FeSO₄·7H₂O with enhanced mass-transfer efficiency, presenting a new strategy to improve the fermentation level of rhamnolipids.

鼠李糖脂是糖脂生物表面活性剂，作为化学合成表面活性剂的清洁替代品具有广泛的应用。由于实现的产量低，生产鼠李糖脂的成本通常很高。这种成本限制了它们在商业应用中的使用。在这项研究中，开发了一种具有高细胞密度的先进连续补料分批发酵工艺，用于高效发酵生产鼠李糖脂。通过这种方法，铜绿假单胞菌产生的总鼠李糖脂超过 665 g/LPAO1 在 18 天的发酵期内，具有 1.54 g/L·h 的极高生产率。这表示比常规补料分批发酵提高了 5 倍以上。由于细胞密度高，18 g/L NaNO ₃和2 mg/L FeSO ₄ ·7H ₂ O的存在，目前先进的连续补料分批发酵工艺非常高效，具有更高的传质效率，提出了一种新的策略提高鼠李糖脂的发酵水平。