Beer spoilage bacteria present severe challenges for the beer fermentation industry. Here, we establish a rapid, specific and highly portable CRISPR/Cas12a-based key beer spoilage bacterial scanning method, dubbed CRISPR-Beer Scan. With the variable 16S rDNA segments of these major spoilage microbe species serving as targets, highly efficient and specific crRNAs were determined. By coupling with recombinase polymerase isothermal amplification, CRISPR-Beer Scan could detect as low as 10 copies of DNA targets. Indeed, trace amounts of target genome DNAs can be accurately identified within a mixture of genome DNAs conditioned in beer. Moreover, the extracted genome DNA samples can be conveniently distinguished through the visual fluorescent signals excited by blue light in 45 min with CRISPR-Beer Scan Method. Taken together, the CRISPR-Beer Scan represents a widely applicable microbe-monitoring platform for not only breweries, but also other industries.

啤酒腐败菌对啤酒发酵行业提出了严峻的挑战。在这里，我们建立了一种快速、特异且高度便携的基于 CRISPR/Cas12a 的关键啤酒腐败细菌扫描方法，称为 CRISPR-Beer Scan。以这些主要腐败微生物物种的可变 16S rDNA 片段为目标，确定了高效和特异的 crRNA。通过与重组酶聚合酶等温扩增相结合，CRISPR-Beer Scan 可以检测低至 10 个拷贝的 DNA 目标。事实上，可以在啤酒中调节的基因组 DNA 混合物中准确识别痕量的目标基因组 DNA。此外，提取的基因组DNA样本可以通过CRISPR-Beer扫描方法在45分钟内通过蓝光激发的视觉荧光信号方便地进行区分。综合起来，