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Decellularization of ram cardiac tissue via supercritical CO2
The Journal of Supercritical Fluids ( IF 3.9 ) Pub Date : 2021-10-16 , DOI: 10.1016/j.supflu.2021.105453
Nevra Pelin Cesur 1 , Nelisa Türkoğlu Laçin 1
Affiliation  

The decellularization method efficacy varies depending on the tissue type. There are commonly used physical, chemical and enzymatic methods. Most of the methods affect the extracellular matrix properties that will in turn alter the cell fate when the biological scaffold is recellularised. We aimed to optimize the decellularization of ram cardiac via scCO2 extraction to preserve the moisture and the components of the ECM during the decellularization procedure. In literature decellularization studies focus on heart vessels. In this study, we aimed to decellularize and recellularize ram cardiac tissue for the first time. Besides we obtained a decellularized tissue without cells on it with a mechanical strength similar to native ECM. Decellularized ram cardiac tissue is expected to be animal-based biomaterial for candidate biomedical applications in vitro and in vivo further studies.



中文翻译:

通过超临界 CO2 对公羊心脏组织进行脱细胞化

脱细胞方法的功效因组织类型而异。常用的有物理法、化学法和酶法。大多数方法都会影响细胞外基质的特性,当生物支架重新细胞化时,这些特性会反过来改变细胞命运。我们旨在通过 scCO 2优化公羊心脏的脱细胞提取以在脱细胞过程中保存 ECM 的水分和成分。在文献中,脱细胞研究侧重于心脏血管。在这项研究中,我们的目标是首次对公羊心脏组织进行去细胞化和再细胞化。此外,我们获得了一个没有细胞的脱细胞组织,其机械强度类似于天然 ECM。脱细胞公羊心脏组织有望成为用于候选生物医学应用的体外和体内进一步研究的动物基生物材料。

更新日期:2021-10-28
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