Reliable analysis of phenolic compounds in fatty matrices is a challenging task. In this work, a robust analytical method was developed and validated for 55 phenolic compounds employing QuEChERS (quick, efficient, cheap, easy, rugged and safe) and Enhanced Matrix Removal (EMR)-lipid cleanup in 96-well plates for sample preparation, coupled with ultra-high performance liquid chromatography with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). Seven high-fat matrices of pork brain, belly and liver; horse serum, beef, salmon and avocado were explored for method validation and led to promising stepwise recoveries of extraction, clean-up, drying-reconstitution of most analytes ranging from 75% to 113%, and with an accuracy of 78%∼117%, except for six catechin-analogues. The matrix removal efficiency of EMR was determined using UHPLC-quadruple time of flight (QTOF)-MS, and results indicated that 56%∼77% of co-extractives were removed. This method would be readily extended to wide range of applications demanding high-throughput and sensitive analysis of phenolic compounds in fatty samples.

对脂肪基质中的酚类化合物进行可靠分析是一项具有挑战性的任务。在这项工作中，采用 QuEChERS（快速、高效、廉价、简单、耐用和安全）和增强型基质去除 (EMR)-脂质净化在 96 孔板中进行样品制备，开发并验证了 55 种酚类化合物的稳健分析方法，结合超高效液相色谱和三重四极杆质谱 (UHPLC-QqQ-MS/MS)。猪脑、猪肚、猪肝七种高脂肪基质；马血清、牛肉、鲑鱼和鳄梨被用于方法验证，并导致大多数分析物的提取、净化、干燥重建的逐步回收率从 75% 到 113% 不等，准确度为 78% 到 117% ，除了六种儿茶素类似物。使用UHPLC-四极杆飞行时间(QTOF)-MS测定EMR的基质去除效率，结果表明去除了56%~77%的共萃取物。这种方法很容易扩展到需要对脂肪样品中的酚类化合物进行高通量和灵敏分析的广泛应用。