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Comparative Proteomics Reveals the Effect of the Transcriptional Regulator Sp13016 on Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2021-10-16 , DOI: 10.1021/acs.jafc.1c03654
Jianli Tang 1 , Haocheng He 1 , Yunlong Li 1 , Zhudong Liu 1 , Ziyuan Xia 1 , Li Cao 1 , Zirong Zhu 1 , Ling Shuai 1 , Yang Liu 1 , Qianqian Wan 1 , Yuewen Luo 1 , Youming Zhang 1 , Jie Rang 1 , Liqiu Xia 1
Affiliation  

Butenyl-spinosyn is a highly effective and broad-spectrum biopesticide produced by Saccharopolyspora pogona. However, the yield of this compound is difficult to increase because the regulatory mechanism of secondary metabolism is still unknown. Here, the transcriptional regulator Sp13016 was discovered to be highly associated with butenyl-spinosyn synthesis and bacterial growth. Overexpression of sp13016 improved butenyl-spinosyn production to a level that was 2.84-fold that of the original strain, while deletion of sp13016 resulted in a significant decrease in yield and growth inhibition. Comparative proteomics revealed that these phenotypic changes were attributed to the influence of Sp13016 on the central carbon metabolism pathway to regulate the supply of precursors. Our research helps to reveal the regulatory mechanism of butenyl-spinosyn biosynthesis and provides a reference for increasing the yield of natural products of Actinomycetes.

中文翻译:

比较蛋白质组学揭示了转录调节因子 Sp13016 对 Saccharopolyspora pogona 中丁烯多杀菌素生物合成的影响

丁烯多杀菌素是一种高效广谱的生物农药,由Saccharopolyspora pogona生产。然而,由于次生代谢的调控机制尚不清楚,该化合物的产量难以提高。在这里,发现转录调节因子 Sp13016 与丁烯多杀菌素合成和细菌生长高度相关。sp13016的过表达将丁烯多杀菌素的产量提高到原始菌株的 2.84 倍,而sp13016的缺失导致产量和生长抑制显着下降。比较蛋白质组学显示,这些表型变化归因于 Sp13016 对中央碳代谢途径以调节前体供应的影响。我们的研究有助于揭示丁烯多杀菌素生物合成的调控机制,为提高放线菌天然产物的产量提供参考。
更新日期:2021-10-27
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