Pyrenophora teres f. teres is a necrotrophic fungal pathogen and causal agent of net form net blotch (NFNB), a significant disease of barley. RNA-seq data encompassing asymptomatic and subsequent necrotrophic phases of the pathogen was obtained for P. teres f. teres isolate W1-1 in NFNB-sensitive cultivar Baudin. Host genes notably regulated during infection included concerted induction of over half the repertoire of disease resistance genes, together with genes involved in oxidation-reduction processes, characteristic of a hypersensitive response. Several systemic acquired resistance response genes were suppressed and there was a complete absence of defense-related thionin gene expression. In P. teres f. teres, genes involved in hydrolase activities and cell-wall catabolic processes were induced during infection, while nitrate assimilation and response to oxidative stress processes were suppressed. Timecourse data allowed a number of predicted P. teres f. teres effector genes with differing expression profiles to be identified that may underlie barley sensitivity to NFNB. Candidate genes involved in the host-pathogen interaction provide a basis for functional characterization and control strategies based on fungicide or mutation targets, which will facilitate further research aimed at controlling NFNB disease.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

Pyrenophora teres f. teres是一种坏死性真菌病原体，也是大麦的一种重要病害——网状网斑病 (NFNB) 的病原体。数据包含病原体的无症状和随后的死体营养阶段是为获得的RNA-SEQ P. TERES F。teres在 NFNB 敏感品种 Baudin 中分离出 W1-1。在感染期间受到显着调节的宿主基因包括协同诱导超过一半的抗病基因库，以及参与氧化还原过程的基因，这是过敏反应的特征。几个系统性获得性抗性反应基因被抑制，完全没有防御相关的硫素基因表达。在P. teres f.teres，参与水解酶活性和细胞壁分解代谢过程的基因在感染过程中被诱导，而硝酸盐同化和对氧化应激过程的反应受到抑制。时间进程数据允许许多预测的P. teres f。大圆效应具有不同的表达谱的基因被鉴定出可依据大麦敏感性NFNB。参与宿主-病原体相互作用的候选基因为基于杀真菌剂或突变目标的功能表征和控制策略提供了基础，这将促进旨在控制 NFNB 疾病的进一步研究。

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