The biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt) is a crucial factor causing reduction in global wheat production. Wild wheat relatives, for example Thinopyrum intermedium, is one of the wild-used parents in wheat disease-resistant breeding. From T. intermedium line, we identified the aspartic protease gene, TiAP1, which is involved in resistance against Bgt. TiAP1 is a secreted protein that accumulates in large amounts at the infection sites of Bgt and extends to the intercellular space. Yeast two-hybrid, luciferase complementation imaging and bimolecular florescent complimentary analysis showed that TiAP1 interacted with the chitin deacetylase (BgtCDA1) of Bgt. The yeast expression, purification and in vitro test confirmed the chitin deacetylase activity of BgtCDA1. The bombardment and VIGS-mediated host-induced gene silencing showed that BgtCDA1 promotes the invasion of Bgt. Transcriptome analysis showed the cell wall xylan metabolism, lignin biosynthesis-related and defence genes involved in the signal transduction were up-regulated in the transgenic TiAP1 wheat induced by Bgt. The TiAP1 in wheat may inactivate the deacetylation function of BgtCDA1, cause chitin oligomers expose to wheat chitin receptor, then trigger the wheat immune response to inhibit the growth and penetration of Bgt, and thereby enhance the resistance of wheat to pathogens.

中文翻译：

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Thiopyrum intermedium TiAP1 与来自 Blumeria graminis f 的几丁质脱乙酰酶相互作用。sp。tritici 并增加小麦对 Bgt 的抗性

生物营养真菌病原体 Blumeria graminis f. sp。tritici ( Bgt ) 是导致全球小麦减产的关键因素。野生小麦近缘种，如中间稻草，是小麦抗病育种中常用的亲本之一。从T。 中间系，我们鉴定了天冬氨酸蛋白酶基因TiAP1，它参与了对Bgt的抗性。TiAP1 是一种分泌蛋白，在Bgt的感染部位大量积累并延伸至细胞间隙。酵母双杂交、荧光素酶互补成像和双分子荧光互补分析表明，TiAP1 与Bgt的几丁质脱乙酰酶 (BgtCDA1) 相互作用。酵母表达、纯化和体外试验证实了BgtCDA1的几丁质脱乙酰酶活性。轰击和VIGS介导的宿主诱导基因沉默表明BgtCDA1促进了Bgt的入侵。转录组分析显示Bgt诱导的转基因TiAP1小麦细胞壁木聚糖代谢、木质素生物合成相关基因和参与信号转导的防御基因上调。. 小麦中的TiAP1可能使BgtCDA1的去乙酰化功能失活，使几丁质寡聚物暴露于小麦几丁质受体，进而引发小麦免疫反应抑制Bgt的生长和渗透，从而增强小麦对病原菌的抵抗力。