当前位置:
X-MOL 学术
›
ACS Appl. Mater. Interfaces
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Enzyme- and Relative Humidity-Responsive Antimicrobial Fibers for Active Food Packaging
ACS Applied Materials & Interfaces ( IF 9.5 ) Pub Date : 2021-10-14 , DOI: 10.1021/acsami.1c12319 Zeynep Aytac 1 , Jie Xu 1 , Suresh Kumar Raman Pillai 2 , Brian D Eitzer 3 , Tao Xu 1 , Nachiket Vaze 1 , Kee Woei Ng 1, 4, 5 , Jason C White 3 , Mary B Chan-Park 2 , Yaguang Luo 6 , Philip Demokritou 1, 4
ACS Applied Materials & Interfaces ( IF 9.5 ) Pub Date : 2021-10-14 , DOI: 10.1021/acsami.1c12319 Zeynep Aytac 1 , Jie Xu 1 , Suresh Kumar Raman Pillai 2 , Brian D Eitzer 3 , Tao Xu 1 , Nachiket Vaze 1 , Kee Woei Ng 1, 4, 5 , Jason C White 3 , Mary B Chan-Park 2 , Yaguang Luo 6 , Philip Demokritou 1, 4
Affiliation
Active food packaging materials that are sustainable, biodegradable, and capable of precise delivery of antimicrobial active ingredients (AIs) are in high demand. Here, we report the development of novel enzyme- and relative humidity (RH)-responsive antimicrobial fibers with an average diameter of 225 ± 50 nm, which can be deposited as a functional layer for packaging materials. Cellulose nanocrystals (CNCs), zein (protein), and starch were electrospun to form multistimuli-responsive fibers that incorporated a cocktail of both free nature-derived antimicrobials such as thyme oil, citric acid, and nisin and cyclodextrin-inclusion complexes (CD-ICs) of thyme oil, sorbic acid, and nisin. The multistimuli-responsive fibers were designed to release the free AIs and CD-ICs of AIs in response to enzyme and RH triggers, respectively. Enzyme-responsive release of free AIs is achieved due to the degradation of selected polymers, forming the backbone of the fibers. For instance, protease enzyme can degrade zein polymer, further accelerating the release of AIs from the fibers. Similarly, RH-responsive release is obtained due to the unique chemical nature of CD-ICs, enabling the release of AIs from the cavity at high RH. The successful synthesis of CD-ICs of AIs and incorporation of antimicrobials in the structure of the multistimuli-responsive fibers were confirmed by X-ray diffraction and Fourier transform infrared spectrometry. Fibers were capable of releasing free AIs when triggered by microorganism-exudated enzymes in a dose-dependent manner and releasing CD-IC form of AIs in response to high relative humidity (95% RH). With 24 h of exposure, stimuli-responsive fibers significantly reduced the populations of foodborne pathogenic bacterial surrogates Escherichia coli (by ∼5 log unit) and Listeria innocua (by ∼5 log unit), as well as fungi Aspergillus fumigatus (by >1 log unit). More importantly, the fibers released more AIs at 95% RH than at 50% RH, which resulted in a higher population reduction of E. coli at 95% RH. Such biodegradable, nontoxic, and multistimuli-responsive antimicrobial fibers have great potential for broad applications as active and smart packaging systems.
中文翻译:
用于活性食品包装的酶和相对湿度响应型抗菌纤维
对可持续、可生物降解且能够精确递送抗菌活性成分 (AI) 的活性食品包装材料的需求量很大。在这里,我们报告了平均直径为 225 ± 50 nm 的新型酶和相对湿度 (RH) 响应性抗菌纤维的开发,该纤维可以沉积为包装材料的功能层。纤维素纳米晶体 (CNC)、玉米醇溶蛋白(蛋白质)和淀粉被静电纺丝以形成多刺激响应纤维,其中掺入了由天然衍生的游离抗菌剂(如百里香油、柠檬酸和乳酸链球菌素)和环糊精包合复合物(CD- ICs) 的百里香油、山梨酸和乳链菌肽。多刺激响应纤维被设计为分别响应酶和 RH 触发器释放游离 AI 和 AI 的 CD-IC。由于所选聚合物的降解,形成了纤维的骨架,从而实现了游离 AI 的酶响应释放。例如,蛋白酶可以降解玉米醇溶蛋白聚合物,进一步加速 AI 从纤维中释放。同样,由于 CD-IC 的独特化学性质,获得了 RH 响应释放,从而能够在高 RH 下从腔中释放 AI。通过 X 射线衍射和傅里叶变换红外光谱法证实了 AI 的 CD-IC 的成功合成和抗菌剂在多刺激响应纤维结构中的掺入。当由微生物分泌的酶以剂量依赖性方式触发时,纤维能够释放游离 AI,并响应高相对湿度(95% RH)释放 CD-IC 形式的 AI。暴露24小时,大肠杆菌(约 5 log 单位)和无害李斯特菌(约 5 log 单位)以及真菌烟曲霉(> 1 log 单位)。更重要的是,纤维在 95% RH 下比在 50% RH 下释放更多的 AI,这导致在 95% RH 下大肠杆菌的数量减少更多。这种可生物降解、无毒和多刺激响应的抗菌纤维具有作为活性和智能包装系统的广泛应用的巨大潜力。
更新日期:2021-10-27
中文翻译:
用于活性食品包装的酶和相对湿度响应型抗菌纤维
对可持续、可生物降解且能够精确递送抗菌活性成分 (AI) 的活性食品包装材料的需求量很大。在这里,我们报告了平均直径为 225 ± 50 nm 的新型酶和相对湿度 (RH) 响应性抗菌纤维的开发,该纤维可以沉积为包装材料的功能层。纤维素纳米晶体 (CNC)、玉米醇溶蛋白(蛋白质)和淀粉被静电纺丝以形成多刺激响应纤维,其中掺入了由天然衍生的游离抗菌剂(如百里香油、柠檬酸和乳酸链球菌素)和环糊精包合复合物(CD- ICs) 的百里香油、山梨酸和乳链菌肽。多刺激响应纤维被设计为分别响应酶和 RH 触发器释放游离 AI 和 AI 的 CD-IC。由于所选聚合物的降解,形成了纤维的骨架,从而实现了游离 AI 的酶响应释放。例如,蛋白酶可以降解玉米醇溶蛋白聚合物,进一步加速 AI 从纤维中释放。同样,由于 CD-IC 的独特化学性质,获得了 RH 响应释放,从而能够在高 RH 下从腔中释放 AI。通过 X 射线衍射和傅里叶变换红外光谱法证实了 AI 的 CD-IC 的成功合成和抗菌剂在多刺激响应纤维结构中的掺入。当由微生物分泌的酶以剂量依赖性方式触发时,纤维能够释放游离 AI,并响应高相对湿度(95% RH)释放 CD-IC 形式的 AI。暴露24小时,大肠杆菌(约 5 log 单位)和无害李斯特菌(约 5 log 单位)以及真菌烟曲霉(> 1 log 单位)。更重要的是,纤维在 95% RH 下比在 50% RH 下释放更多的 AI,这导致在 95% RH 下大肠杆菌的数量减少更多。这种可生物降解、无毒和多刺激响应的抗菌纤维具有作为活性和智能包装系统的广泛应用的巨大潜力。