Background Soleris® Direct Yeast and Mold (DYM) is a growth-based, automated method for detection of yeast and mold in select foods and other sample types including nutraceuticals and cosmetics. The Soleris method is used in a “dilute-to-specification” or threshold manner in which a result is scored as positive or negative around a predetermined cutoff (in CFU/g) established by the dilution and volume of sample homogenate tested. Objective The objective of this study was to validate the method for testing of dried cannabis flower. The validation was conducted under the Emergency Response Validation program of the AOAC Research Institute. Method The study included inclusivity and exclusivity testing, in particular testing of yeast and mold species associated with cannabis, and a matrix study in which Soleris method presumptive results were compared with Soleris confirmed results using Dichloran Rose-Bengal Chloramphenicol (DRBC) agar for confirmation. Samples at four different levels of natural yeast and mold contamination were tested at two test thresholds. Results In inclusivity testing, all 63 yeast and mold strains tested produced positive results within the specified test duration of 72 h. In exclusivity testing, 36 of 37 strains tested produced no detection within 72 h. In matrix testing, there were no significant differences between Soleris presumptive and confirmed results for any contamination level or test threshold as determined by probability of detection analysis. Conclusions Results indicate that the Soleris method is an effective procedure for detection of yeast and mold in dried cannabis flower. Highlights With the Soleris method, results are available within 72 h compared with the 5–7 days required for microbiological culture methods.

中文翻译：

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用于检测干大麻花中酵母和霉菌的 Soleris® 直接酵母和霉菌方法的应急响应验证：AO​​AC 性能测试方法 SM 051301

背景 Soleris® Direct Yeast and Mold (DYM) 是一种基于生长的自动化方法，用于检测精选食品和其他样品类型（包括保健品和化妆品）中的酵母和霉菌。Soleris 方法以“按规格稀释”或阈值方式使用，其中在通过所测样品匀浆的稀释和体积确定的预定截止值（以 CFU/g 为单位）附近将结果评分为阳性或阴性。目的本研究的目的是验证干大麻花的检测方法。该验证是在 AOAC 研究所的应急响应验证计划下进行的。方法 该研究包括包容性和排他性测试，特别是与大麻相关的酵母和霉菌种类的测试，以及一项基质研究，其中将 Soleris 方法的推定结果与 Soleris 确认的结果进行比较，使用二氯兰玫瑰-孟加拉氯霉素 (DRBC) 琼脂进行确认。在两个测试阈值下测试了四种不同水平的天然酵母和霉菌污染的样品。结果 在包容性测试中，所有 63 种酵母和霉菌菌株都在规定的 72 小时测试时间内产生了阳性结果。在排他性测试中，测试的 37 个菌株中有 36 个在 72 小时内未检测到。在基质测试中，Soleris 推定结果和确认结果对于任何污染水平或测试阈值之间没有显着差异，由检测概率分析确定。结论 结果表明，Soleris 法是检测大麻干花中酵母菌和霉菌的有效方法。