Dysregulated long noncoding RNAs (lncRNAs) have potential roles in various cancer types. The objective of this study was to investigate the expression and the underlying role of long intergenic nonprotein coding RNA 115 (LINC00115) in lung cancer. The relative expression of LINC00115 and miR-607 in tumor tissues and cells was detected by real-time PCR. After overexpression or knockdown of LINC00115 expression in tumor cells, the changes in the proliferation, migration, and invasion capacities were detected via Counting Kit-8 (CCK-8) assay and transwell assays. The interplay among LINC00115, miR-607, and integrin β1 (ITGB1) was confirmed by bioinformatics analyses and luciferase reporter assay. In addition, tumor cells with LINC00115 knockdown were injected into nude mice to investigate the effect of LINC00115 on tumorigenesis in vivo. LINC00115 was highly expressed in tumor tissues and cells. LINC00115 promoted the malignant properties of tumor cells. Investigation to its molecular mechanism revealed that LINC00115 functioned as a competitive endogenous RNA (ceRNA), regulating the expression of ITGB1 by sponging miR-607 to affect tumor growth. The LINC00115/miR-607/ITGB1 signaling axis might be a novel therapeutic target in lung cancer.

中文翻译：

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LncRNA LINC00115 通过 miR-607/ITGB1 通路促进肺癌进展

失调的长链非编码 RNA (lncRNA) 在各种癌症类型中具有潜在作用。本研究的目的是调查长基因间非蛋白编码 RNA 115 (LINC00115) 在肺癌中的表达和潜在作用。实时荧光定量PCR检测LINC00115和miR-607在肿瘤组织和细胞中的相对表达量。在肿瘤细胞中过表达或敲低 LINC00115 表达后，通过 Counting Kit-8 (CCK-8) 测定和 transwell 测定检测增殖、迁移和侵袭能力的变化。LINC00115、miR-607和整合素β1（ITGB1）之间的相互作用通过生物信息学分析和荧光素酶报告基因测定得到证实。此外，将LINC00115敲低的肿瘤细胞注射到裸鼠体内，以研究LINC00115对体内肿瘤发生的影响。LINC00115在肿瘤组织和细胞中高表达。LINC00115促进了肿瘤细胞的恶性特性。对其分子机制的研究表明，LINC00115 作为一种竞争性内源 RNA (ceRNA)，通过海绵 miR-607 影响肿瘤生长来调节 ITGB1 的表达。LINC00115/miR-607/ITGB1 信号轴可能是肺癌的新治疗靶点。