Charge detection mass spectrometry (CDMS) is a single-particle technique where the masses of individual ions are determined from simultaneous measurement of their mass-to-charge ratio (m/z) and charge. Masses are determined for thousands of individual ions, and then the results are binned to give a mass spectrum. Using this approach, accurate mass distributions can be measured for heterogeneous and high-molecular-weight samples that are usually not amenable to analysis by conventional mass spectrometry. Recent applications include heavily glycosylated proteins, protein complexes, protein aggregates such as amyloid fibers, infectious viruses, gene therapies, vaccines, and vesicles such as exosomes.

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电荷检测质谱在分子生物学和生物技术中的应用

电荷检测质谱 (CDMS) 是一种单粒子技术，其中单个离子的质量是通过同时测量它们的质荷比 ( m / z ) 和电荷来确定的。确定数千个单独离子的质量，然后将结果合并以提供质谱。使用这种方法，可以测量通常不适合通过常规质谱分析的异质和高分子量样品的精确质量分布。最近的应用包括高度糖基化的蛋白质、蛋白质复合物、蛋白质聚集体（例如淀粉样蛋白纤维）、传染性病毒、基因疗法、疫苗和囊泡（例如外泌体）。