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A simple and innovative sample preparation method for on-site SARS-CoV-2 molecular diagnostics
Analyst ( IF 4.2 ) Pub Date : 2021-09-17 , DOI: 10.1039/d1an01401c Songhyun Lee 1 , Junkyu Song 2 , Sanghyo Kim 1
Analyst ( IF 4.2 ) Pub Date : 2021-09-17 , DOI: 10.1039/d1an01401c Songhyun Lee 1 , Junkyu Song 2 , Sanghyo Kim 1
Affiliation
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Nucleic acid amplification is a widely used diagnostic tool, although it requires a relatively time-consuming and complicated extraction step. To address this issue outside the laboratory, we investigated a sample preparation system and determined that a silica membrane and silica-coated beads are powerful tools for the extraction from raw samples: nucleic acids are kept in the silica membrane, retained during a single wash step, and released at the elution step. The eluent is appropriate for the quantitative real-time polymerase chain reaction (qPCR) and loop-mediated amplification (LAMP) assay in terms of purity and quantity. We also built an innovative equipment-free nucleic acid extraction squeeze system which requires less than 20 min. The sample with improved purity augments the specificity and sensitivity. This system is simple, user-friendly, low-cost, and equipment-free, thus making nucleic acid extraction more accessible and affordable for researchers and untrained users. Furthermore, when combined with the reverse-transcription quantitative real-time polymerase chain reaction method, the method will accelerate the detection of diseases. The same goes when combined with the LAMP assay, especially in developing countries.
更新日期：2021-10-13
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