Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environmental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDI-TOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have on MALDI-TOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.

中文翻译：

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样品制备和培养条件对 MALDI-TOF MS 细菌鉴定的影响：综述

基质辅助激光解吸/电离飞行时间质谱 (MALDI-TOF MS) 是细菌鉴定的绝佳工具。它可在临床诊断和环境研究中实现高通量、灵敏且特定的应用。目前，对于细菌分析的样品制备和培养条件还没有最佳的标准化方案。MALDI-TOF MS 的性能受多个变量的影响，例如样品制备、培养基和培养条件、孵育时间/生长阶段、孵育温度、高盐含量、培养基中的血液等。因此，本综述旨在澄清为什么统一的方案不合理，评估这些因素对 MALDI-TOF MS 识别评分的影响，并讨论与特定细菌代表和菌株要求相关的方法的可能优化。