Here, we report the complete genome sequence of a novel polerovirus, "Plantago asiatica virus A" (PlaVA), detected in Plantago asiatica using high-throughput RNA sequencing and validated by Sanger sequencing. The complete PlaVA genome contains 5,881 nucleotides and has seven open reading frames (ORF0-5 and ORF3a) encoding putative proteins (P0-5 and P3a, respectively) in an arrangement that is similar to that of typical Polerovirus members. Pairwise sequence comparisons revealed that P0 to P5 encoded by PlaVA had the highest sequence identity (25.48%-79.21%) to the corresponding proteins of previously reported poleroviruses. A phylogenetic analysis using the PlaVA P1-2 and P3 amino acid sequences and those of members of the family Solemoviridae (formerly Luteoviridae) indicated that although PlaVA belongs to the genus Polerovirus, it does not represent a known species. Consequently, PlaVA should be considered a member of a new species within the genus Polerovirus.

中文翻译：

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车前子亚洲病毒 A 的完整基因组序列，一种新的假定成员 Polerovirus。

在这里，我们报告了使用高通量 RNA 测序在车前草中检测到并通过 Sanger 测序验证的新型杆状病毒“车前草病毒 A”(PlaVA) 的完整基因组序列。完整的 PlaVA 基因组包含 5,881 个核苷酸，并具有 7 个开放阅读框（ORF0-5 和 ORF3a），它们编码假定的蛋白质（分别为 P0-5 和 P3a），其排列类似于典型的 Polerovirus 成员。成对序列比较显示，PlaVA 编码的 P0 到 P5 与先前报道的杆状病毒的相应蛋白质具有最高的序列同一性 (25.48%-79.21%)。使用 PlaVA P1-2 和 P3 氨基酸序列以及梭状病毒科（以前称为黄体病毒科）成员的系统发育分析表明，虽然 PlaVA 属于 Polerovirus 属，它不代表已知物种。因此，PlaVA 应该被认为是 Polerovirus 属中一个新物种的成员。