Identifying the causal pathogen of encephalitis remains a clinical challenge. A 50-year-old man without a history of neurological disease was referred to our department for the evaluation of an intracranial lesion observed on brain magnetic resonance imaging (MRI) scans, and the pathology results suggested protozoal infection. We identified the species responsible for encephalitis using thymine-adenine (TA) cloning, suitable for routine clinical practice.

中文翻译：

---

用通用真核引物克隆胸腺嘧啶-腺嘌呤诊断颧骨脑炎。

确定脑炎的致病病原体仍然是一个临床挑战。一名没有神经系统疾病史的 50 岁男性被转诊到我科，以评估在脑磁共振成像 (MRI) 扫描中观察到的颅内病变，病理结果提示原虫感染。我们使用适合常规临床实践的胸腺嘧啶腺嘌呤 (TA) 克隆确定了导致脑炎的物种。