Low temperatures result in poor anaerobic digestion (AD). To investigate whether bioaugmentation can improve anaerobic co-digestion of cattle manure and corn straw at 20 °C, five different doses of methanogenic propionate-degrading culture (4%, 8%, 12%, 14%, and 16%) were added to batch AD systems to compare bioaugmentation performance. The results showed that the methane production of all the bioaugmented digesters was enhanced compared to the control, increasing 2.80–4.20-fold with digestion times (T₈₀) shorter by 11–22 d. The recommended dose for biogas production was 14%, and the recommended dose for the highest bioaugmentation efficiency of microbes was 4%. These improvements were due to the addition of methanogenic propionate-degrading culture, which alleviated volatile fatty acids (VFA) accumulation, especially that of acetate and propionate. Metagenomic sequencing analysis indicated that the increased proportion of propionate-oxidizing bacteria, syntrophic butyrate-oxidizing bacteria, and acetoclastic methanogens in bioaugmentation reactors may be responsible for better AD performance.

低温会导致厌氧消化 (AD) 不良。为了研究生物强化是否可以改善 20 °C 下牛粪和玉米秸秆的厌氧共消化，将五种不同剂量的产甲烷丙酸盐降解培养物（4%、8%、12%、14% 和 16%）添加到批量 AD 系统来比较生物强化性能。结果表明，与对照相比，所有生物增强消化器的甲烷产量均有所提高，随着消化时间的增加（T ₈₀) 缩短了 11-22 天。生产沼气的推荐剂量为14%，微生物最高生物强化效率的推荐剂量为4%。这些改进是由于添加了产甲烷丙酸盐降解培养物，这减轻了挥发性脂肪酸 (VFA) 的积累，尤其是醋酸盐和丙酸盐的积累。宏基因组测序分析表明，生物强化反应器中丙酸氧化细菌、同养丁酸氧化细菌和乙酰碎屑产甲烷菌的比例增加可能是更好的 AD 性能的原因。