The lignin-first biorefinery approach was desirable to produce lignin-derived products by protecting the linkages of lignin and reducing condensation reaction. However, so far lignin-first strategy studies were mainly carried out under harsh conditions, causing serious destruction of lignin structure and reduction of chemically labile linkages, which was not conducive to enhance value of lignin adequately. In this work, mild fractionation of poplar via lignin-first strategy using dioxane/methanol at 80 °C was developed for purposely extracting reactive lignin with a relatively higher yield (>50%), purity (>99%), β-O-4′ linkages and p-hydroxybenzoate group as compared with controlled sample. In addition, glucose yield of cellulose-rich residue under lignin-first strategy was significantly enhanced to 98.57% due to the superior cellulase adsorption abilities, which was obviously higher than the controlled group (53.88%). Overall, this mild lignin-first strategy was promising to fractionate lignocellulose into reactive lignin and fermentable glucose, thereby achieving full utilization of lignocellulose biomass.

通过保护木质素的连接和减少缩合反应来生产木质素衍生产品，木质素优先的生物精炼方法是可取的。然而，迄今为止，木质素优先策略的研究主要是在苛刻的条件下进行，导致木质素结构的严重破坏和化学不稳定键的减少，不利于充分提高木质素的价值。在这项工作中，开发了在 80 °C 下使用二恶烷/甲醇通过木质素优先策略对杨树进行温和分馏，以有目的地提取具有相对较高产率 (>50%)、纯度 (>99%)、β-O-的活性木质素。 4' 连接和p-羟基苯甲酸酯组与对照样品相比。此外，由于纤维素酶的吸附能力优越，木质素优先策略下富含纤维素的残留物的葡萄糖产率显着提高至98.57％，明显高于对照组（53.88％）。总体而言，这种温和的木质素优先策略有望将木质纤维素分馏为活性木质素和可发酵葡萄糖，从而实现木质纤维素生物质的充分利用。