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Towards valorization of pectin-rich agro-industrial residues: Engineering of Saccharomyces cerevisiae for co-fermentation of d-galacturonic acid and glycerol
Metabolic Engineering ( IF 8.4 ) Pub Date : 2021-10-12 , DOI: 10.1016/j.ymben.2021.10.001
Andreea Perpelea 1 , Andy Wiranata Wijaya 2 , Luís C Martins 3 , Dorthe Rippert 1 , Mathias Klein 1 , Angel Angelov 4 , Kaisa Peltonen 5 , Attila Teleki 6 , Wolfgang Liebl 7 , Peter Richard 5 , Johan M Thevelein 8 , Ralf Takors 6 , Isabel Sá-Correia 9 , Elke Nevoigt 1
Affiliation  

Pectin-rich plant biomass residues represent underutilized feedstocks for industrial biotechnology. The conversion of the oxidized monomer d-galacturonic acid (d-GalUA) to highly reduced fermentation products such as alcohols is impossible due to the lack of electrons. The reduced compound glycerol has therefore been considered an optimal co-substrate, and a cell factory able to efficiently co-ferment these two carbon sources is in demand. Here, we inserted the fungal d-GalUA pathway in a strain of the yeast S. cerevisiae previously equipped with an NAD-dependent glycerol catabolic pathway. The constructed strain was able to consume d-GalUA with the highest reported maximum specific rate of 0.23 g gCDW−1 h−1 in synthetic minimal medium when glycerol was added. By means of a 13C isotope-labelling analysis, carbon from both substrates was shown to end up in pyruvate. The study delivers the proof of concept for a co-fermentation of the two ‘respiratory’ carbon sources to ethanol and demonstrates a fast and complete consumption of d-GalUA in crude sugar beet pulp hydrolysate under aerobic conditions. The future challenge will be to achieve co-fermentation under industrial, quasi-anaerobic conditions.



中文翻译:

实现富含果胶的农工业残留物的价值化:酿酒酵母工程用于 d-半乳糖醛酸和甘油的共同发酵

富含果胶的植物生物质残留物代表了工业生物技术未充分利用的原料。由于缺乏电子,氧化单体d-半乳糖醛酸 ( d -GalUA) 无法转化为高度还原的发酵产物,例如醇类。因此,还原的化合物甘油被认为是一种最佳的辅助底物,并且需要能够有效地共同发酵这两种碳源的细胞工厂。在这里,我们将真菌d -GalUA 途径插入到以前配备有 NAD 依赖性甘油分解代谢途径的酵母S. cerevisiae菌株中。所构建的菌株能够消耗d -GalUA,最高报道的最大比率为 0.23 gg添加甘油时,合成基本培养基中的CDW -1 h -1 。通过13 C 同位素标记分析,两种底物的碳都显示为丙酮酸。该研究提供了将两种“呼吸性”碳源共同发酵成乙醇的概念证明,并证明了在有氧条件下粗制甜菜浆水解产物中的d -GalUA 的快速和完全消耗。未来的挑战将是在工业、准厌氧条件下实现共发酵。

更新日期:2021-10-29
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