Abstract

This study aimed to evaluate the in vitro effect of aloe emodin, barbaloin and chrysophanol on growing and mature biofilms of Cryptococcus neoformans sensu stricto. The compounds were added at the moment of inducing biofilm growth or after growth for 72 h to evaluate their effects on growing and mature biofilms, respectively. Then, biofilm biomass was evaluated by crystal violet staining and metabolic activity by the XTT reduction assay. Morphological alterations were also evaluated by laser scanning confocal microscopy. Aloe emodin and barbaloin affected growing biofilms and disrupted mature biofilms, reducing metabolic activity by > 60% and biomass by > 70%. Chrysophanol only inhibited mature biofilms, but to a lesser extent. In conclusion, anthraquinones, especially aloe emodin and barbaloin, show a relevant effect against growing and mature biofilms of C. neoformans sensu stricto.

摘要

本研究旨在评估芦荟大黄素、大黄素和大黄酚对严格意义上的新型隐球菌生长和成熟生物膜的体外作用。. 在诱导生物膜生长的时刻或在生长 72 小时后加入这些化合物，分别评估它们对生长和成熟生物膜的影响。然后，通过结晶紫染色和 XTT 还原测定法评估生物膜生物量。还通过激光扫描共聚焦显微镜评估了形态学改变。芦荟大黄素和巴巴洛因影响生长的生物膜并破坏成熟的生物膜，使代谢活性降低 > 60%，生物量降低 > 70%。大黄酚仅抑制成熟的生物膜，但程度较轻。总之，蒽醌类化合物，尤其是芦荟大黄素和巴巴洛因，对新生隐球菌 sensu stricto 的生长和成熟生物膜显示出相关作用。