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Hydroxyl Radical Protein Footprinting: A Mass Spectrometry-Based Structural Method for Studying the Higher Order Structure of Proteins
Chemical Reviews ( IF 62.1 ) Pub Date : 2021-10-11 , DOI: 10.1021/acs.chemrev.1c00432
Alan McKenzie-Coe 1 , Nicholas S Montes 1 , Lisa M Jones 1
Affiliation  

Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been successfully used to investigate a plethora of protein-related questions. The method, which utilizes hydroxyl radicals to oxidatively modify solvent-accessible amino acids, can inform on protein interaction sites and regions of conformational change. Hydroxyl radical-based footprinting was originally developed to study nucleic acids, but coupling the method with mass spectrometry has enabled the study of proteins. The method has undergone several advancements since its inception that have increased its utility for more varied applications such as protein folding and the study of biotherapeutics. In addition, recent innovations have led to the study of increasingly complex systems including cell lysates and intact cells. Technological advances have also increased throughput and allowed for better control of experimental conditions. In this review, we provide a brief history of the field of HRPF and detail recent innovations and applications in the field.

中文翻译:

羟基自由基蛋白质足迹:一种基于质谱的结构方法研究蛋白质的高阶结构

羟基自由基蛋白质足迹 (HRPF) 与质谱联用已成功用于研究大量与蛋白质相关的问题。该方法利用羟基自由基对溶剂可及的氨基酸进行氧化修饰,可以告知蛋白质相互作用位点和构象变化区域。基于羟基自由基的足迹最初是为了研究核酸而开发的,但将该方法与质谱法相结合已经能够研究蛋白质。该方法自问世以来经历了多项进步,增加了其在蛋白质折叠和生物治疗学研究等更多应用中的实用性。此外,最近的创新导致对包括细胞裂解物和完整细胞在内的日益复杂的系统的研究。技术进步也提高了产量,并允许更好地控制实验条件。在这篇评论中,我们简要介绍了 HRPF 领域的历史,并详细介绍了该领域的最新创新和应用。
更新日期:2021-10-11
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