Although poly(ethylene glycol) (PEG) is commonly used in nanoparticle design, the impact of surface topography on nanoparticle performance in biomedical applications has received little attention, despite showing significant promise in the study of inorganic nanoparticles. Control of the surface topography of polymeric nanoparticles is a formidable challenge due to the limited conformational control of linear polymers that form the nanoparticle surface. In this work, we establish a straightforward method to precisely tailor the surface topography of PEGylated polymeric nanoparticles based on tuning the architecture of shape-persistent amphiphilic bottlebrush block copolymer (BBCP) building blocks. We demonstrate that nanoparticle formation and surface topography can be controlled by systematically changing the structural parameters of BBCP architecture. Furthermore, we reveal that the surface topography of PEGylated nanoparticles significantly affects their performance. In particular, the adsorption of a model protein and the uptake into HeLa cells were closely correlated to surface roughness and BBCP terminal PEG block brush width. Overall, our work elucidates the importance of surface topography in nanoparticle research as well as provides an approach to improve the performance of PEGylated nanoparticles.

中文翻译：

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由瓶刷嵌段共聚物形成的聚乙二醇壳纳米颗粒的表面形貌控制与蛋白质和细胞的相互作用

尽管聚（乙二醇）（PEG）通常用于纳米颗粒设计，但表面形貌对生物医学应用中纳米颗粒性能的影响却很少受到关注，尽管在无机纳米颗粒的研究中显示出巨大的前景。由于形成纳米颗粒表面的线性聚合物的构象控制有限，因此控制聚合物纳米颗粒的表面形貌是一项艰巨的挑战。在这项工作中，我们建立了一种简单的方法来精确定制聚乙二醇化聚合物纳米粒子的表面形貌，该方法基于调整形状持久性两亲性瓶刷嵌段共聚物 (BBCP) 构建块的结构。我们证明了纳米粒子的形成和表面形貌可以通过系统地改变 BBCP 结构的结构参数来控制。此外，我们揭示聚乙二醇化纳米粒子的表面形貌显着影响其性能。特别是，模型蛋白的吸附和 HeLa 细胞的吸收与表面粗糙度和 BBCP 末端 PEG 块刷宽度密切相关。总的来说，我们的工作阐明了表面形貌在纳米粒子研究中的重要性，并提供了一种提高聚乙二醇化纳米粒子性能的方法。模型蛋白的吸附和 HeLa 细胞的吸收与表面粗糙度和 BBCP 末端 PEG 块刷宽度密切相关。总的来说，我们的工作阐明了表面形貌在纳米粒子研究中的重要性，并提供了一种提高聚乙二醇化纳米粒子性能的方法。模型蛋白的吸附和 HeLa 细胞的吸收与表面粗糙度和 BBCP 末端 PEG 块刷宽度密切相关。总的来说，我们的工作阐明了表面形貌在纳米粒子研究中的重要性，并提供了一种提高聚乙二醇化纳米粒子性能的方法。