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MdWRKY75e enhances resistance to Alternaria alternata in Malus domestica
Horticulture Research ( IF 8.7 ) Pub Date : 2021-10-11 , DOI: 10.1038/s41438-021-00701-0
Yingjun Hou 1 , Xinyi Yu 1 , Weiping Chen 2 , Weibing Zhuang 3 , Sanhong Wang 1 , Chao Sun 1 , Lifang Cao 1 , Tingting Zhou 1 , Shenchun Qu 1
Affiliation  

The Alternaria alternata apple pathotype adversely affects apple (Malus domestica Borkh.) cultivation. However, the molecular mechanisms underlying enhanced resistance to this pathogen in apple remain poorly understood. We have previously reported that MdWRKY75 expression is upregulated by A. alternata infection in ‘Sushuai’ apples. In this study, we discovered that overexpression of MdWRKY75e increased the resistance of transgenic apple lines to A. alternata infection, whereas silencing this gene enhanced susceptibility to A. alternata infection. Furthermore, we found that MdWRKY75e directly binds to the MdLAC7 promoter to regulate the biosynthesis of laccase and increase the biosynthesis of lignin during A. alternata infection. Moreover, the thickening of the cell wall enhanced the mechanical defense capabilities of apple. In addition, we found that jasmonic acid remarkably induced MdWRKY75e expression, and its levels in transgenic apple lines were elevated. These results indicate that MdWRKY75e confers resistance to the A. alternata apple pathotype mainly via the jasmonic acid pathway and that pathogenesis-related genes and antioxidant-related enzyme activity are involved in the disease resistance of MdWRKY75e transgenic plants. In conclusion, our findings provide insights into the importance of MdWRKY75e for resistance to A. alternata infection in apples.

中文翻译:

MdWRKY75e 增强了苹果对链格孢的抗性

交链格孢苹果病型对苹果有不利影响(海棠Borkh.) 栽培。然而,苹果对这种病原体的抗性增强的分子机制仍然知之甚少。我们之前曾报道过MdWRKY75表达被上调A.alternata感染“苏帅”苹果。在这项研究中,我们发现过度表达MdWRKY75e增加了转基因苹果系的抗性A.alternata感染,而沉默该基因增强了对感染的易感性A.alternata感染。此外,我们发现 MdWRKY75e 直接与MdLAC7启动子调节漆酶的生物合成并增加木质素的生物合成A.alternata感染。此外,细胞壁的增厚增强了苹果的机械防御能力。此外,我们发现茉莉酸显着诱导MdWRKY75e表达,并且其在转基因苹果系中的水平升高。这些结果表明MdWRKY75e赋予抵抗力A.alternata苹果致病型主要通过茉莉酸途径,发病相关基因和抗氧化相关酶活性参与了苹果的抗病性。MdWRKY75e转基因植物。总之,我们的研究结果提供了洞察力的重要性MdWRKY75e为抵抗A.alternata苹果感染。
更新日期:2021-10-11
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