Clitopilus hobsonii (Entolomataceae, Agaricales, Basidiomycetes) is a common soil saprotroph. There is also evidence that C. hobsonii can act as a root endophyte benefitting tree growth. Here, we report the genome assembly of C. hobsonii QYL-10, isolated from ectomycorrhizal root tips of Quercus lyrata. The genome size is 36.93 Mb, consisting of 13 contigs (N₅₀ = 3.3 Mb) with 49.2% GC content. Of them, 10 contigs approached the length of intact chromosomes, and three had telomeres at one end only. BUSCO analysis reported a completeness score of 98.4%, using Basidiomycota_odb10 lineage data. Combining ab-initio, RNA-seq data, and homology-based predictions, we identified 12,710 protein-coding genes. Approximately, 1.43 Mb of transposable elements (3.88% of the assembly), 36 secondary metabolite biosynthetic gene clusters, and 361 genes encoding putative carbohydrate-active enzymes were identified. This genomic resource will allow functional studies aimed to characterize the symbiotic interactions between C. hobsonii and its host trees and will also provide a valuable foundation for further research on comparative genomics of the Entolomataceae.

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Clitopilus hobsonii (Entolomataceae, Agaricales, Basidiomycetes) 是一种常见的土壤腐生菌。还有证据表明C. hobsonii可以作为有利于树木生长的根内生菌。在这里，我们报告了从Quercus lyrata外生菌根根尖分离的C. hobsonii QYL-10的基因组组装。基因组大小为 36.93 Mb，由 13 个重叠群（N ₅₀ = 3.3 Mb）组成，GC 含量为 49.2%。其中，10个contigs接近完整染色体的长度，3个只有一端有端粒。BUSCO 分析报告使用担子菌_odb10 谱系数据的完整性得分为 98.4%。从头结合、RNA-seq 数据和基于同源性的预测，我们确定了 12,710 个蛋白质编码基因。大约确定了 1.43 Mb 的转座元件（组装的 3.88%）、36 个次级代谢物生物合成基因簇和 361 个编码假定碳水化合物活性酶的基因。这种基因组资源将允许功能研究旨在表征C. hobsonii与其宿主树之间的共生相互作用，并且还将为进一步研究 Entolomataceae 的比较基因组学提供宝贵的基础。

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