In recent years, various methods for detecting exogenous and endogenous hypochlorite have been studied, considering its essential role as a biomolecule. However, the existing technologies still pose obstacles such as their invasiveness, high costs, and complicated operation. In the current study, we developed a glow-type chemiluminescent probe, hypochlorite chemiluminescence probe (HCCL)-1, based on the scaffold of Schaap's 1,2-dioxetane luminophores. To better explore the physiological and pathological functions of hypochlorite, we modified the luminophore scaffold of HCCL-1 to develop several probes, including HCCL-2, HCCL-3, and HCCL-4, which amplify the response signal of hypochlorite. By comparing the luminescent intensities of the four probes using the IVIS® system, we determined that HCCL-2 with a limit of detection of 0.166 μM has enhanced sensitivity and selectivity for tracking hypochlorite both in vitro and in vivo.

近年来，考虑到其作为生物分子的重要作用，已经研究了各种检测外源性和内源性次氯酸盐的方法。然而，现有技术仍存在侵入性强、成本高、操作复杂等障碍。在目前的研究中，我们基于 Schaap 的 1,2-二氧杂环丁烷发光体的支架开发了一种发光型化学发光探针，即次氯酸盐化学发光探针 (HCCL)-1。为了更好地探索次氯酸盐的生理和病理功能，我们对 HCCL-1 的发光支架进行了改造，开发了几种探针，包括 HCCL-2、HCCL-3 和 HCCL-4，它们可以放大次氯酸盐的响应信号。通过使用 IVIS® 系统比较四种探针的发光强度，我们确定 HCCL-2 的检测限为 0。