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Characteristics of the Gut Microbiota and Metabolism in Patients With Latent Autoimmune Diabetes in Adults: A Case-Control Study
Diabetes Care ( IF 16.2 ) Pub Date : 2021-12-01 , DOI: 10.2337/dc20-2975
Yuanyuan Fang 1 , Chenhong Zhang 2 , Hongcai Shi 3 , Wei Wei 1 , Jing Shang 1 , Ruizhi Zheng 1 , Lu Yu 1 , Pingping Wang 1 , Junpeng Yang 1 , Xinru Deng 1 , Yun Zhang 1 , Shasha Tang 1 , Xiaoyang Shi 1 , Yalei Liu 1 , Huihui Yang 1 , Qian Yuan 1 , Rui Zhai 4 , Huijuan Yuan 5
Affiliation  

OBJECTIVE

Type 1 and type 2 diabetes are associated with gut dysbiosis. However, the relationship between the gut microbiota and latent autoimmune diabetes in adults (LADA), sharing clinical and metabolic features with classic type 1 and type 2 diabetes, remains unclear. Here, we used a multiomics approach to identify the characteristics of the gut microbiota and metabolic profiles in patients with LADA.

RESEARCH DESIGN AND METHODS

This age- and sex-matched case-control study included 30 patients with LADA, 31 patients with classic type 1 diabetes, 30 patients with type 2 diabetes, and 29 healthy individuals. The gut microbiota profiles were identified through the 16S rRNA gene, and fecal and serum metabolites were measured through untargeted liquid chromatography-mass spectrometry.

RESULTS

Patients with LADA had a significantly different structure and composition of the gut microbiota and their metabolites as well as a severe deficiency of short-chain fatty acid–producing bacteria. The gut microbiota structure of the patients with LADA was more similar to that of patients with type 1 diabetes who were positive for GAD antibody. We identified seven serum metabolite modules and eight fecal metabolite modules that differed between the LADA group and the other groups.

CONCLUSIONS

The characteristic gut microbiota and related metabolites of patients with LADA are associated with autoantibodies, glucose metabolism, islet function, and inflammatory factors, which may contribute to the pathogenesis of LADA. Future longitudinal studies should explore whether modulating the gut microbiota and related metabolites can alter the natural course of autoimmune diabetes in the quest for new therapeutics.



中文翻译:

成人潜伏性自身免疫性糖尿病患者肠道微生物群和代谢的特征:病例对照研究

客观的

1 型和 2 型糖尿病与肠道菌群失调有关。然而,肠道微生物群与成人潜伏性自身免疫性糖尿病 (LADA) 之间的关系,与经典的 1 型和 2 型糖尿病具有相同的临床和代谢特征,仍不清楚。在这里,我们使用多组学方法来确定 LADA 患者肠道微生物群的特征和代谢特征。

研究设计与方法

这项年龄和性别匹配的病例对照研究包括 30 名 LADA 患者、31 名经典 1 型糖尿病患者、30 名 2 型糖尿病患者和 29 名健康个体。通过 16S rRNA 基因鉴定肠道微生物群谱,并通过非靶向液相色谱-质谱法测量粪便和血清代谢物。

结果

LADA 患者的肠道微生物群及其代谢物的结构和组成明显不同,并且严重缺乏产生短链脂肪酸的细菌。LADA 患者的肠道菌群结构与 GAD 抗体阳性的 1 型糖尿病患者更相似。我们确定了 LADA 组和其他组之间不同的 7 个血清代谢物模块和 8 个粪便代谢物模块。

结论

LADA 患者的特征性肠道菌群及相关代谢物与自身抗体、糖代谢、胰岛功能和炎症因子有关,可能与 LADA 的发病机制有关。未来的纵向研究应该探索调节肠道微生物群和相关代谢物是否可以改变自身免疫性糖尿病的自然病程以寻求新的治疗方法。

更新日期:2021-12-07
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