Wound healing involves a complex series of events where cell–cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity in vitro and angiogenesis in ex ovo chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in ex ovo CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.

中文翻译：

---

去细胞的细胞外基质装饰的 PCL PolyHIPE 支架可增强细胞活性、整合和血管生成

伤口愈合涉及一系列复杂的事件，其中细胞 - 细胞和细胞 - 细胞外基质 (ECM) 相互作用起着关键作用。伤口可以很简单，例如上皮完整性的丧失，也可以更深更复杂，到达皮下组织，包括血管、肌肉和神经。伤口区域的快速新生血管形成对于伤口愈合至关重要，因为它在高要求的增殖阶段和炎症细胞向伤口区域的轮回中提供氧气和营养方面具有关键作用。一种避免延迟新生血管形成的方法是外源性使用促血管生成因子，这是昂贵的、高度依赖剂量的，并且它们的递送需要非常良好控制的系统以避免渗漏、高渗透性和出血性血管形成。在这项研究中，体外和离体小鸡绒毛尿囊膜 (CAM) 测定中的血管生成。我们的结果表明，支架中包含 ECM 增加了三种类型细胞的代谢活性，这些细胞在 7 天的体外CAM 测定中在伤口愈合和刺激血管生成中起关键作用。在此，我们证明了成纤维细胞-ECM 功能化的 PCL PolyHIPE 支架似乎具有用作活性伤口敷料以促进血管生成和伤口愈合的巨大潜力。