This study aimed to evaluate the effect of supercritical CO₂ process for the inactivation of artificially contaminated seeds of leek and alfalfa. The seeds were inoculated with E. coli O157:H7, Listeria monocytogenes, and Salmonella Thompson and S. Typhimurium and treated at 80 and 120 bar and at 35 and 45 °C for 20 min. The process did not influence the germination rate of the seeds. The inactivation was dependent from the type of seed and pressure and temperature. At 120 bar and 45 °C E. coli O157:H7, L. monocytogenes, and Salmonella spp. were reduced by 2.92 ± 0.27, 1.14 ± 0.63, and 1.74 ± 0.55 log CFU/g, respectively, on alfalfa and by 4.96 ± 0.37, 2.93 ± 0.27, and 3.18 ± 0.27, respectively on leek. Overall, these results indicated that supercritical CO₂ can be used to improve the microbial safety of sprouts, especially for leek.

本研究旨在评估超临界 CO ₂工艺对人为污染的韭菜和苜蓿种子的灭活效果。将种子接种大肠杆菌O157：H7，单增李斯特菌，和沙门氏菌Thompson和S.鼠伤寒沙门氏菌，并在80和120巴处理，并在35和45℃下20分钟。该过程不影响种子的发芽率。灭活取决于种子的类型以及压力和温度。在 120 bar 和 45 °C 下，大肠杆菌O157:H7、单核细胞增生李斯特氏菌和沙门氏菌属 在苜蓿上分别降低了 2.92 ± 0.27、1.14 ± 0.63 和 1.74 ± 0.55 log CFU/g，在韭菜上分别降低了 4.96 ± 0.37、2.93 ± 0.27 和 3.18 ± 0.27。总体而言，这些结果表明超临界 CO ₂可用于提高芽的微生物安全性，尤其是韭菜。