The effectiveness of hydrolysis and acidification via liquid fraction of digestate (LFD-HA) strategy on enhancing methane production of corn straw (CS) were explored in this study. A post-hydrolysis and acidification (HA) process was applied to treating CS by adding LFD to the acidogenic reactor to improve microbial pre-decomposition and further solubilize recalcitrant organic polymers. Through combining the LFD and HA modification, the process was upgraded with associated improvement in anaerobic digestion (AD) performance. The variations of physico-chemical structure and AD performance of CS after LFD-HA compared with NaOH modification were analyzed. The results evidenced that LFD-HA promoted degradation of lignin monomer compounds from macromolecular polymers to aromatic compounds and acetic acid. The lignocellulosic structures and linkages were destroyed by hydroxy radical and hydrolytic enzyme. Compared with CS without pretreatment, the highest methane yield of LFD-HA CS was 265.1 mL·g⁻¹VS⁻¹, and the digestion time (T₈₀) was shortened by 27.8%. The populations of Methanobacteriaceae, Methanosarcinaceae and Ruminococcaceae were enriched during LFD-HA process. This research proposed that LFD-HA was an efficient and eco-friendly process in improving lignocellulose degradation and bio-methane generation from CS.

本研究探讨了通过消化液液体部分 (LFD-HA) 策略进行水解和酸化对提高玉米秸秆 (CS) 甲烷产量的有效性。通过向产酸反应器中加入 LFD 以改善微生物的预分解并进一步溶解顽固的有机聚合物，将后水解和酸化 (HA) 工艺应用于处理 CS。通过将 LFD 和 HA 改进相结合，该工艺得到了升级，厌氧消化 (AD) 性能得到了相关改进。分析了LFD-HA与NaOH改性后CS的理化结构和AD性能的变化。结果表明，LFD-HA 促进了木质素单体化合物从大分子聚合物降解为芳香族化合物和乙酸。木质纤维素的结构和连接被羟基自由基和水解酶破坏。与未经预处理的CS相比，LFD-HA CS的最高甲烷产率为265.1 mL·g^-1 VS ^-1，消化时间（T ₈₀）缩短了27.8%。LFD-HA过程富集了甲烷杆菌科、甲烷八叠球菌科和瘤胃球菌科的种群。该研究表明，LFD-HA 是一种有效且环保的工艺，可改善木素纤维素降解和 CS 产生的生物甲烷。