• Components of the plant immune signaling network need mechanisms that confer resilience against fast-evolving pathogen effectors that target them. Among eight Arabidopsis CaM-Binding Protein (CBP) 60 family members, AtCBP60g and AtSARD1 are partially functionally redundant, major positive immune regulators, and AtCBP60a is a negative immune regulator. We investigated possible resilience-conferring evolutionary mechanisms among the CBP60a, CBP60g and SARD1 immune regulatory subfamilies.
• Phylogenetic analysis was used to investigate the times of CBP60 subfamily neofunctionalization. Then, using the pairwise distance rank based on the newly developed analytical platform Protein Evolution Analysis in a Euclidean Space (PEAES), hypotheses of specific coevolutionary mechanisms that could confer resilience on the regulator module were tested.
• The immune regulator subfamilies diversified around the time of angiosperm divergence and have been evolving very quickly. We detected significant coevolutionary interactions across the immune regulator subfamilies in all of 12 diverse core eudicot species lineages tested. The coevolutionary interactions were consistent with the hypothesized coevolution mechanisms.
• Despite their unusually fast evolution, members across the CBP60 immune regulator subfamilies have influenced the evolution of each other long after their diversification in a way that could confer resilience on the immune regulator module against fast-evolving pathogen effectors.

中文翻译：

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病原体驱动的 CBP60 植物免疫调节子亚科共同进化赋予调节器模块弹性

• 植物免疫信号网络的组成部分需要能够对针对它们的快速进化的病原体效应物赋予弹性的机制。在 8 个拟南芥 CaM 结合蛋白 (CBP) 60 家族成员中，AtCBP60g 和 AtSARD1 是部分功能冗余的主要正向免疫调节因子，而 AtCBP60a 是负向免疫调节因子。我们研究了 CBP60a、CBP60g 和 SARD1 免疫调节亚家族之间可能赋予弹性的进化机制。
• 系统发育分析用于研究CBP60亚家族新功能化的时间。然后，使用基于新开发的分析平台欧几里得空间中的蛋白质进化分析 (PEAES) 的成对距离等级，测试了可以赋予调节器模块弹性的特定共同进化机制的假设。
• 免疫调节亚科在被子植物分化时期出现多样化，并且发展非常迅速。我们在测试的所有 12 个不同的核心真双子叶植物谱系中检测到免疫调节亚科的显着共同进化相互作用。共同进化的相互作用与假设的共同进化机制一致。
• 尽管 CBP60 免疫调节子家族的成员进化异常迅速，但在其多样化之后很长时间内，它们仍然影响了彼此的进化，这种方式可以赋予免疫调节模块抵御快速进化的病原体效应物的能力。