Background Eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are proteins released by activated eosinophils whose role in adult asthma remains unclear. Objective To study associations between ECP, EDN and various asthma characteristics in adults from the Epidemiological Study on the Genetics and Environment of Asthma (EGEA). Methods Plasma ECP and EDN levels were measured by ELISA. Cross-sectional analyses were performed in 941 adults (43±16 years old, 39% with asthma) at EGEA2 (2003–2007). Longitudinal analyses investigated the associations between EDN level at EGEA2 and changes in asthma characteristics between EGEA2 and EGEA3 (2011–2013, n=817). We used generalised estimated equations adjusted for age, sex, smoking status and body mass index to take into account familial dependence. Results At EGEA2, both high ECP and EDN levels were associated with current asthma (adjusted OR (aOR) (95% CI): 1.69 (1.35–2.12) and 2.12 (1.76–2.57)). Among asthmatics, high EDN level was associated with asthma attacks (aOR: 1.50 (1.13–1.99)), wheezing and breathlessness (aOR: 1.38 (1.05–1.80)), use of asthma treatments (aOR: 1.91 (1.37–2.68)) and bronchial hyper-responsiveness (aOR: 2.03 (1.38–2.97)), even after further adjustment on ECP. High ECP level was associated with high neutrophil count and tended to be associated with chronic bronchitis. High EDN level at EGEA2 was associated with persistent asthma (aOR: 1.62 (1.04–2.52)), nocturnal symptoms (aOR from 2.19 to 3.57), worsening wheezing and breathlessness (aOR: 1.97 (1.36–2.85)) and nocturnal shortness of breath (aOR: 1.44 (1.04–1.98)) between EGEA2 and EGEA3. Conclusions EDN and ECP were associated with different asthma expression in adults. EDN could be a potential biomarker to monitor asthma evolution in adults. No data are available. Due to third-party restrictions, EGEA data are not publicly available. Please see the following URL for more information: . Interested researchers should contact egea.cohorte@inserm.fr with further questions regarding data access.

中文翻译：

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血液嗜酸性粒细胞阳离子蛋白和嗜酸性粒细胞衍生的神经毒素与成人不同的哮喘表达和演变有关

背景 嗜酸性粒细胞衍生的神经毒素 (EDN) 和嗜酸性粒细胞阳离子蛋白 (ECP) 是由活化的嗜酸性粒细胞释放的蛋白质，其在成人哮喘中的作用仍不清楚。目的通过哮喘遗传与环境流行病学研究（EGEA）研究ECP、EDN与成人哮喘各种特征的关系。方法采用ELISA法测定血浆ECP和EDN水平。在 EGEA2（2003-2007 年）对 941 名成年人（43±16 岁，39% 患有哮喘）进行了横断面分析。纵向分析调查了 EGEA2 的 EDN 水平与 EGEA2 和 EGEA3 之间哮喘特征变化之间的关联（2011-2013，n=817）。我们使用根据年龄、性别、吸烟状况和体重指数调整的广义估计方程来考虑家庭依赖性。结果在 EGEA2，高 ECP 和 EDN 水平均与当前哮喘相关（调整后的 OR (aOR) (95% CI)：1.69 (1.35–2.12) 和 2.12 (1.76–2.57)）。在哮喘患者中，高 EDN 水平与哮喘发作 (aOR: 1.50 (1.13–1.99))、喘息和呼吸困难 (aOR: 1.38 (1.05–1.80))、使用哮喘治疗 (aOR: 1.91 (1.37–2.68)) 相关和支气管高反应性（aOR：2.03 (1.38–2.97)），即使在 ECP 进一步调整后。高 ECP 水平与高中性粒细胞计数相关，并且往往与慢性支气管炎相关。EGEA2 的高 EDN 水平与持续性哮喘（aOR：1.62 (1.04–2.52)）、夜间症状（aOR 从 2.19 到 3.57）、喘息和呼吸困难恶化（aOR：1.97 (1.36–2.85)）和夜间呼吸急促有关(aOR: 1.44 (1.04–1.98)) 在 EGEA2 和 EGEA3 之间。结论 EDN和ECP与成人不同哮喘表达相关。EDN 可能是监测成人哮喘演变的潜在生物标志物。没有可用的数据。由于第三方限制，EGEA 数据不公开。有关详细信息，请参阅以下 URL：. 感兴趣的研究人员应联系 egea.cohorte@inserm.fr 以了解有关数据访问的更多问题。