Resource discovery for producing biologically active phytochemicals plays an important role in industrial crops area. Ingenol-3-angelate, one of the most concerned macrocyclic diterpenoids, has been approved for clinical treatment of actinic keratosis and industrially produced by a semi-synthesis approach using hydrolysis products of ingenol derivatives. Ingenol derivatives are considered exclusive among Euphorbia genus at a quite low level. In search for high-ingenol plant species from the Euphorbiaceae family, ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) was performed for the quantitative analysis of the free ingenol content in the hydrolyzed methanol extract of 61 selected plant species, including 50 Euphorbia plants, 9 plants from other Euphorbiaceae genera, and 2 Phyllanthaceae plants. The ingenol derivatives in E. milii and E. cotinifolia which contained high level of ingenol esters were further identified by UHPLC-MS. Amounts of ingenol diterpenes varied among the samples. The highest free ingenol content (527.82 ± 38.19 μmol/kg of dry herb weight) was found in a variation, E. tithymaloides 'Nanus', followed by E. tithymaloides (402.81 ± 28.89 μmol/kg), E. milii (391.30 ± 43.51 μmol/kg), E. ammak (379.75 ± 34.68 μmol/kg), and E. trigona (357.07 ± 34.29 μmol/kg). The results revealed the trace amount of ingenol derivatives in some Jatropha, Acalypha, Codiaeum, and Excoecaria plants for the first time and confirmed the higher level of ingenol esters in some Euphorbia plants cultivated under polycarbonate panel with higher UV transmission than those grown under double layer glass. The secondary metabolites varied distinctively among different Euphorbia sections. This work suggested more applicable sources for ingenol production and better greenhouse covering material for Euphorbia plants cultivation in consideration of ingenol derivatives.

生产具有生物活性的植物化学物质的资源发现在经济作物领域发挥着重要作用。Ingenol-3-angelate 是最受关注的大环二萜类化合物之一，已被批准用于光化性角化病的临床治疗，并通过使用大戟醇衍生物的水解产物的半合成方法进行工业化生产。大戟属植物中的Ingenol 衍生物被认为在相当低的水平上是排他性的。为寻找大戟科高大戟科植物物种，采用超高效液相色谱-质谱联用技术（UHPLC-MS）定量分析了61种选定植物的水解甲醇提取物中的游离大戟醇含量，包括 50大戟植物，大戟科其他属植物 9 株，叶兰科植物 2 株。UHPLC-MS 进一步鉴定了E. milii和E. cotinifolia中含有高水平大戟醇酯的大戟醇衍生物。不同样品中大戟二萜的含量不同。在变种E. tithymaloides 'Nanus' 中发现了最高的游离大姜醇含量（527.82 ± 38.19 μmol/kg 干草本重量），其次是E. tithymaloides (402.81 ± 28.89 μmol/kg)、E. milii (391.30 ± 43.51 μmol/kg)、E. ammak (379.75 ± 34.68 μmol/kg) 和E. trigona (357.07 ± 34.29 μmol/kg)。结果表明，某些产品中含有微量的大戟醇衍生物。首次对麻风树、木瓜、木耳属和Excoecaria植物进行了验证，并证实了在紫外线透射率高于双层玻璃下种植的一些大戟属植物中，一些大戟属植物中的大戟醇酯含量更高。不同大戟属植物的次生代谢产物差异显着。考虑到大戟醇衍生物，这项工作提出了更适用的大戟醇生产来源和更好的大戟植物栽培温室覆盖材料。