当前位置: X-MOL 学术Microb. Genom. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A genomic epidemiological study shows that prevalence of antimicrobial resistance in Enterobacterales is associated with the livestock host, as well as antimicrobial usage
Microbial Genomics ( IF 3.9 ) Pub Date : 2021-10-05 , DOI: 10.1099/mgen.0.000630
Manal AbuOun 1 , Hannah Jones 2 , Emma Stubberfield 1 , Daniel Gilson 2 , Liam P Shaw 3 , Alasdair T M Hubbard 3, 4 , Kevin K Chau 3 , Robert Sebra 5 , Tim E A Peto 3 , Derrick W Crook 3, 6 , Daniel S Read 7 , H Soon Gweon 7, 8 , A Sarah Walker 3, 6 , Nicole Stoesser 3, 6 , Richard P Smith 2 , Muna F Anjum 1 , On Behalf Of The Rehab Consortium
Affiliation  

Enterobacterales from livestock are potentially important reservoirs for antimicrobial resistance (AMR) to pass through the food chain to humans, thereby increasing the AMR burden and affecting our ability to tackle infections. In this study 168 isolates from four genera of the order   Enterobacterales  , primarily   Escherichia coli  , were purified from livestock (cattle, pigs and sheep) faeces from 14 farms in the United Kingdom. Their genomes were resolved using long- and short-read sequencing to analyse AMR genes and their genetic context, as well as to explore the relationship between AMR burden and on-farm antimicrobial usage (AMU), in the three months prior to sampling. Although   E. coli   isolates were genomically diverse, phylogenetic analysis using a core-genome SNP tree indicated pig isolates to generally be distinct from sheep isolates, with cattle isolates being intermediates. Approximately 28 % of isolates harboured AMR genes, with the greatest proportion detected in pigs, followed by cattle then sheep; pig isolates also harboured the highest number of AMR genes per isolate. Although 90 % of sequenced isolates harboured diverse plasmids, only 11 % of plasmids (n=58 out of 522) identified contained AMR genes, with 91 % of AMR plasmids being from pig, 9 % from cattle and none from sheep isolates; these results indicated that pigs were a principle reservoir of AMR genes harboured by plasmids and likely to be involved in their horizontal transfer. Significant associations were observed between AMU (mg kg−1) and AMR. As both the total and the numbers of different antimicrobial classes used on-farm increased, the risk of multi-drug resistance (MDR) in isolates rose. However, even when AMU on pig farms was comparatively low, pig isolates had increased likelihood of being MDR; harbouring relatively more resistances than those from other livestock species. Therefore, our results indicate that AMR prevalence in livestock is not only influenced by recent AMU on-farm but also livestock-related factors, which can influence the AMR burden in these reservoirs and its plasmid mediated transmission.

中文翻译:

一项基因组流行病学研究表明,肠杆菌属中抗菌素耐药性的流行与家畜宿主以及抗菌素的使用有关

来自牲畜的肠杆菌可能是抗菌素耐药性 (AMR) 通过食物链传递给人类的重要宿主,从而增加了 AMR 负担并影响我们应对感染的能力。在这项研究中,从英国 14 个农场的牲畜(牛、猪和羊)粪便中纯化了来自四个 肠杆菌 属的 168 个分离物,主要是 大肠杆菌 。他们的基因组在采样前三个月使用长读长和短读长测序来分析 AMR 基因及其遗传背景,并探索 AMR 负担与农场抗生素使用 (AMU) 之间的关系。虽然 大肠杆菌       分离株在基因组上是多样化的,使用核心基因组 SNP 树的系统发育分析表明猪分离株通常与绵羊分离株不同,而牛分离株是中间体。大约 28% 的分离株携带 AMR 基因,在猪中检测到的比例最大,其次是牛,然后是羊;猪分离株的每个分离株的 AMR 基因数量也最多。尽管 90% 的测序分离株含有不同的质粒,但只有 11% 的质粒(n= 522 个中的 58 个)鉴定含有 AMR 基因,其中 91% 的 AMR 质粒来自猪,9% 来自牛,没有来自绵羊分离株;这些结果表明,猪是质粒携带的 AMR 基因的主要储存库,并且可能参与其水平转移。AMU (mg kg -1) 和 AMR。随着农场使用的不同抗菌药物种类的总数和数量的增加,分离株中多重耐药性(MDR)的风险也随之上升。然而,即使养猪场的 AMU 相对较低,猪分离株成为 MDR 的可能性也会增加;比其他牲畜物种具有相对更多的抵抗力。因此,我们的研究结果表明,牲畜中 AMR 的流行不仅受最近农场 AMU 的影响,还受到与牲畜相关的因素的影响,这些因素可以影响这些水库中的 AMR 负担及其质粒介导的传播。
更新日期:2021-10-06
down
wechat
bug