Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) are available. In this study, we report the complete sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) along with the construction of an infectious full-length cDNA clone via Gibson assembly. The sequence of CVYV Portugal shows the closest relationship to a CVYV isolate from Spain (genome, 99.7% identity; polyprotein, 99.7% identity). The CVYV full-length cDNA clone was introduced by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, resulting in symptoms resembling those of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This first report confirming the infectivity of a CVYV cDNA clone provides the opportunity to study gene functions in a consistent genomic background.

黄瓜叶脉黄化病毒 (CVYV) 是马铃薯病毒科 Ipomovirus 属的成员。在国家生物技术信息中心 (NCBI) 数据库中，可以获得来自西班牙 (NC_006941)、以色列 (KT276369) 和约旦 (JF460793) 的 CVYV 分离株的三个完整基因组序列。在这项研究中，我们报告了来自葡萄牙的 CVYV 分离株 (DSMZ PV-0776) 的完整序列以及通过 Gibson 组装构建的感染性全长 cDNA 克隆。CVYV Portugal 的序列与西班牙的 CVYV 分离株关系最密切（基因组，99.7% 同一性；多蛋白，99.7% 同一性）。CVYV 全长 cDNA 克隆通过电穿孔导入放射根瘤菌并渗入黄瓜幼苗，导致类似于野生型病毒的症状。证实了由粉虱Bemisia tabaci传播传染性 CVYV 全长克隆。第一份确认 CVYV cDNA 克隆的感染性的报告提供了在一致的基因组背景中研究基因功能的机会。