We have designed and synthesized a multifunctional dendritic molecular probe that selectively detects Cu²⁺ ions via potentiometric and fluorometric techniques with low detection limits (3.5 μM in potentiometry, 15 nM in fluorometry). The selective and reversible binding of the molecule with the Cu²⁺ ion was used to make a solid-state microsensor (diameter of 25 μm) by incorporating the molecular probe into the carbon-based membrane as an ionophore for Cu(II). The Cu(II) microelectrode has a broad linear range of 10 μM to 1 mM with a near Nernstian slope of 30 mV/log [a_Cu²⁺] and detection limit of 3.5 μM. The Cu(II) microsensor has a fast response time (1.5 s), and it has a broad working pH range from 3.5 to 6.0. The incorporation of the hydrophobic dendritic moiety makes the ionophore less prone to leaching in an aqueous matrix for potentiometric measurement. The cinnamaldehyde component of the molecule helps detection of Cu²⁺ ions fluorometrically, as indicated by a change in fluorescence upon selective and reversible binding of the molecular probe to the Cu²⁺ ions. The strategic design of the molecular probe allows us to detect Cu²⁺ ions in drinking water by using this novel dendritic fluoroionophore and solid-state Cu²⁺ – ion-selective microelectrode.

中文翻译：

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使用电位和荧光技术选择性检测 Cu2+ 离子的多功能树枝状分子探针

我们设计并合成了一种多功能树枝状分子探针，可通过电位计和荧光计技术选择性检测 Cu ²⁺离子，检测限低（电位计 3.5 μM，荧光计 15 nM）。该分子与 Cu ²⁺离子的选择性和可逆结合用于通过将分子探针作为 Cu( II )的离子载体掺入碳基膜中来制作固态微传感器（直径为 25 μm ）。Cu( II ) 微电极具有 10 μM 至 1 mM 的宽线性范围，具有 30 mV/log [ a _Cu ²⁺ ] 的近能斯特斜率和 3.5 μM 的检测限。铜（Ⅱ) 微传感器具有快速响应时间（1.5 秒），并且具有从 3.5 到 6.0 的宽工作 pH 范围。疏水树枝状部分的加入使离子载体不易浸出在用于电位测量的水性基质中。分子的肉桂醛成分有助于荧光检测 Cu ^{2+离子，如分子探针与 Cu 2+}离子选择性和可逆结合时的荧光变化所示。分子探针的战略设计使我们能够通过使用这种新型树枝状荧光离子载体和固态 Cu ²⁺ - 离子选择性微电极来检测饮用水中的 Cu ^2+离子。