Here, 55 genes encoding NAC were identified in the jujube genome. Based on gene structures, protein motifs, and phylogenetic relationships, the jujube NAC family was classified into five groups and 15 subgroups. All ZjNAC genes were distributed on 12 chromosomes and four tandem duplication events were detected. The publicly available RNA-Seq data revealed that ZjNAC genes were differentially expressed in various tissues, but a few genes were highly expressed in fruit. Target genes of NAC were predicted and these genes were enriched in stress responses, catabolism and hydrolysis process. Analyses of the cis-acting elements, homology relationship and genes expression levels under abiotic stresses showed that several genes, such as ZjNAC6, ZjNAC35, ZjNAC47 and ZjNAC55, may be identified to be involved in responses to abscisic acid (ABA) and cold, drought, and salt stresses. Additionally, the differences in expression levels between the young and mature tissues imply ZjNAC genes may respond to tissue senescence. These results could lay a solid foundation for investigations of the biological roles of NAC, breeding improvement for stress resistance and retarding senescence in jujube.

在这里，在枣基因组中鉴定了 55 个编码 NAC 的基因。根据基因结构、蛋白质基序和系统发育关系，将枣 NAC 家族分为 5 个组和 15 个亚组。所有ZjNAC基因分布在 12 条染色体上，检测到 4 个串联重复事件。公开的 RNA-Seq 数据显示ZjNAC基因在各种组织中差异表达，但少数基因在果实中高表达。预测了NAC的靶基因，这些基因在应激反应、分解代谢和水解过程中富集。对非生物胁迫下顺式作用元件、同源关系和基因表达水平的分析表明，ZjNAC6等几个基因、ZjNAC35、ZjNAC47和ZjNAC55可能被鉴定为参与对脱落酸 (ABA) 和寒冷、干旱和盐胁迫的响应。此外，年轻和成熟组织之间表达水平的差异意味着ZjNAC基因可能对组织衰老有反应。这些结果可为研究NAC的生物学作用、抗逆育种改良和延缓枣衰老奠定坚实的基础。