Consumption of prawns as a protein source has been on the rise worldwide with seafood identified as the predominant attributable source of human vibriosis. However, surveillance of non-cholera Vibrio is limited both in public health and in food. Using a population- and market share-weighted study design, 211 prawn samples were collected and cultured for Vibrio spp. Contamination was detected in 46 % of samples, and multiple diverse Vibrio isolates were obtained from 34 % of positive samples. Whole genome sequencing (WGS) and phylogenetic analysis illustrated a comprehensive view of Vibrio species diversity in prawns available at retail, with no known pathogenicity markers identified in Vibrio parahaemolyticus and V. cholerae . Antimicrobial resistance genes were found in 77 % of isolates, and 12 % carried genes conferring resistance to three or more drug classes. Resistance genes were found predominantly in V. parahaemolyticus , though multiple resistance genes were also identified in V. cholerae and V. vulnificus . This study highlights the large diversity in Vibrio derived from prawns at retail, even within a single sample. Although there was little evidence in this study that prawns are a major source of vibriosis in the UK, surveillance of non-cholera Vibrio is very limited. This study illustrates the value of expanding WGS surveillance efforts of non-cholera Vibrios in the food chain to identify critical control points for food safety through the production system and to determine the full extent of the public health impact.

中文翻译：

---

全基因组测序揭示零售对虾中弧菌的多样性

对虾作为蛋白质来源的消费在全球范围内呈上升趋势，海产品被确定为人类弧菌病的主要来源。然而，非霍乱弧菌的监测在公共卫生和食品方面都是有限的。使用人口和市场份额加权研究设计，收集并培养了 211 份虾样本以检测弧菌。在 46% 的样本中检测到污染，并且从 34% 的阳性样本中获得了多种不同的弧菌分离株。全基因组测序 (WGS) 和系统发育分析展示了零售对虾弧菌物种多样性的全面视图，在副溶血弧菌中没有发现已知的致病性标记 和霍乱弧菌。在 77% 的分离物中发现了抗菌素耐药基因，12% 的菌株携带了对三种或更多药物类别产生耐药性的基因。抗性基因主要在副溶血性弧菌中发现，但在霍乱弧菌和创伤弧菌中也发现了多种抗性基因。这项研究强调了零售对虾产生的弧菌的巨大多样性，即使在单个样本中也是如此。尽管这项研究中几乎没有证据表明对虾是英国弧菌病的主要来源，但对非霍乱弧菌的监测 非常有限。本研究说明了扩大 WGS 对食物链中非霍乱弧菌的监测工作的价值，以通过生产系统确定食品安全的关键控制点，并确定公共卫生影响的全部程度。