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Elastic property of sickle cell anemia and sickle cell trait red blood cells
Journal of Biomedical Optics ( IF 3.5 ) Pub Date : 2021-09-01 , DOI: 10.1117/1.jbo.26.9.096502
Endris Muhammed 1 , James Cooper 2 , Daniel Devito 2 , Robert Mushi 3 , Maria Del Pilar Aguinaga 3, 4 , Daniel Erenso 2 , Horace Crogman 5
Affiliation  

Significance: We introduce a model for better calibration of the trapping force using an equal but oppositely directed drag force acting on a trapped red blood cell (RBC). We demonstrate this approach by studying RBCs’ elastic properties from deidentified sickle cell anemia (SCA) and sickle cell trait (SCT) blood samples. Aim: A laser trapping (LT) force was formulated and analytically calculated in a cylindrical model. Using this trapping force relative percent difference, the maximum (longitudinal) and minimum (transverse) radius rate and stiffness were used to study the elasticity. Approach: The elastic property of SCA and SCT RBCs was analyzed using LT technique with computer controlled piezo-driven stage, in order to trap and stretch the RBCs. Results: For all parameters, the results show that the SCT RBC samples have higher elastic property than the SCA RBCs. The higher rigidity in the SCA cell may be due to the lipid composition of the membrane, which was affected by the cholesterol concentration. Conclusions: By developing a theoretical model for different trapping forces, we have also studied the elasticity of RBCs in SCT (with hemoglobin type HbAS) and in SCA (with hemoglobin type HbSS). The results for the quantities describing the elasticity of the cells consistently showed that the RBCs in the SCT display lower rigidity and higher deformability than the RBCs with SCA.

中文翻译:

镰状细胞性贫血和镰状细胞性状红细胞的弹性特性

意义:我们引入了一种模型,可以使用作用于被捕获的红细胞 (RBC) 的相等但方向相反的阻力来更好地校准捕获力。我们通过研究来自未鉴定的镰状细胞性贫血 (SCA) 和镰状细胞性状 (SCT) 血液样本的红细胞的弹性特性来证明这种方法。目的:在圆柱模型中制定并分析计算激光捕获 (LT) 力。利用这种捕获力相对百分比差异,使用最大(纵向)和最小(横向)半径率和刚度来研究弹性。方法:使用计算机控制压电驱动台的 LT 技术分析 SCA 和 SCT 红细胞的弹性特性,以捕获和拉伸红细胞。结果:对于所有参数,结果表明 SCT RBC 样品比 SCA RBC 具有更高的弹性。SCA细胞的较高刚性可能是由于细胞膜的脂质成分受到胆固醇浓度的影响。结论:通过开发不同捕获力的理论模型,我们还研究了 SCT(血红蛋白型 HbAS)和 SCA(血红蛋白型 HbSS)中红细胞的弹性。描述细胞弹性的量结果一致表明,SCT 中的红细胞比 SCA 中的红细胞表现出更低的刚性和更高的变形能力。
更新日期:2021-09-29
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