Artificial membranes for investigation of the human absorption (oral, dermal or respiratory) of target organic compounds are aimed at mimicking the interactions occurring at and within the cell lipid membrane. Biomolecules such as proteins are also integral components of the lipid membranes and play a pivotal role towards understanding the complex mechanisms of human absorption. In this review, we will differentiate biomimetic platforms based on static (batchwise) and dynamic modes. In the former, a synthetic membrane placed between two phases (donor and acceptor) mimics a given biological system to study permeability. Parallel artificial membrane permeation assays are the most common approaches for static mode. As to dynamic modes, there is a plethora of bioanalytical techniques such as immobilized artificial membrane chromatography, biopartitioning micellar chromatography or immobilized plasma protein chromatography. In any case, all of the dynamic approaches capitalize upon analytical separation techniques such as liquid chromatography and the use of the chromatographic factors to predict permeability and other bioparameters. However, improvements in the fabrication of novel sorptive materials or the development of innovative techniques/approaches to enhance the prediction capability of permeability by simulated membranes has been left in the background. For this reason, this review covers the current state-of-the-art of immobilized artificial membranes in bioanalytical science with particular focus on new materials and techniques reported from 2015 to mid-2021. Future perspectives related to the fabrication of innovative artificial membranes for in vitro intestinal absorption studies have been highlighted so as to encourage fundamental studies in this research area.

用于研究人体对目标有机化合物吸收（口腔、皮肤或呼吸）的人工膜旨在模拟细胞脂质膜处和细胞脂膜内发生的相互作用。蛋白质等生物分子也是脂质膜的组成部分，在理解人体吸收的复杂机制方面发挥着关键作用。在这篇评论中，我们将根据静态（批量）和动态模式区分仿生平台。在前者中，放置在两相（供体和受体）之间的合成膜模拟给定的生物系统来研究渗透性。平行人工膜渗透试验是静态模式最常用的方法。至于动态模式，有大量的生物分析技术，如固定化人工膜层析、生物分配胶束层析或固定化血浆蛋白层析。在任何情况下，所有动态方法都利用分析分离技术，例如液相色谱和使用色谱因子来预测渗透性和其他生物参数。然而，在制造新型吸附材料或开发创新技术/方法以提高模拟膜渗透率的预测能力方面的改进一直处于背景中。因此，本综述涵盖了生物分析科学中目前最先进的固定化人工膜，特别关注 2015 年至 2021 年中期报告的新材料和技术。与制造创新人造膜相关的未来前景体外肠道吸收研究已得到强调，以鼓励该研究领域的基础研究。