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Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey
International Microbiology ( IF 3.1 ) Pub Date : 2021-09-24 , DOI: 10.1007/s10123-021-00212-x
Sahin Namli 1 , Yesim Soyer 1
Affiliation  

The emerging situation of Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as Salmonella genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) and ability to cause human cases is a consequence of their possession. Screening of SGI1 (and its variants) and C1 integrons was done with conventional PCR, while screening of gene cassettes and virulence genes was conducted with real-time PCR for 70 S. Infantis isolates from poultry products. SGI1 or its variants were not detected in any of the isolates. Sixty-eight of 70 isolates were detected to carry one C1 integron of size 1.0 kb. These integrons were detected to carry ant(3″)-Ia gene cassette explaining the streptomycin/spectinomycin resistance. Sequence analysis of gene cassettes belongs to four representing isolates which showed that, although their difference in isolation date and place, genetically, they are 99.9% similar. Virulence gene screening was introduced as genotypic virulence profiles. The most dominant profile for S. Infantis isolates, among twelve genes, was gatC-tcfA, which are known to be related to colonization at specific hosts. This study revealed the high percentage of C1 integron possession in S. Infantis isolates from poultry products in Turkey. It also showed the potential of S. Infantis strains to be resistant to more antimicrobial drugs. Moreover, a dominant profile of virulence genes that are uncommon for non-typhoidal Salmonella (NTS) serovars was detected, which might explain the enhanced growth at specified hosts.



中文翻译:

土耳其婴儿沙门氏菌血清型1类整合子和毒力基因的调查

沙门氏菌亚种的出现情况。肠炎沙门婴儿(小号在土耳其。婴儿)的毒力基因和可移动的遗传元件,如方面进行了研究沙门氏菌基因组岛1(SGI1)和1级(C1)整合,以查看是否增加多药耐药性(MDR)和能力,原因人类案件是他们拥有的结果。SGI1(及其变体)和C1整合的筛选用常规PCR完成,而基因盒和毒力基因的筛选用实时PCR进行70小号. Infantis 从家禽产品中分离出来。在任何分离株中均未检测到 SGI1 或其变体。检测到 70 个分离株中的 68 个携带一个大小为 1.0 kb 的 C1 整合子。检测到这些整合子携带ant(3")-Ia基因盒,解释了链霉素/壮观霉素抗性。基因盒的序列分析属于四个代表分离株,表明虽然它们的分离日期和地点不同,但在遗传上却有 99.9% 的相似性。引入毒力基因筛选作为基因型毒力谱。S 的主要配置文件。Infantis 分离株,在十二个基因中,是gatC - tcfA,已知这与特定宿主的定植有关。这项研究揭示了S中 C1 整合子占有率很高。Infantis 从土耳其的家禽产品中分离出来。这也显示了S的潜力。婴儿菌株对更多的抗菌药物具有抗药性。此外,检测到非伤寒沙门氏菌(NTS) 血清型不常见的毒力基因的显性特征,这可能解释了特定宿主的生长增强。

更新日期:2021-09-28
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