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Molecular Characterization of a Tolerant Saline-Alkali Chlorella Phosphatidate Phosphatase That Confers NaCl and Sorbitol Tolerance
Frontiers in Microbiology ( IF 5.2 ) Pub Date : 2021-09-28 , DOI: 10.3389/fmicb.2021.738282
Jingang Wang 1 , Qinghua Shan 1 , Ye Ran 1 , Dexiang Sun 1 , Haizhen Zhang 1 , Jinzhu Zhang 1 , Shufang Gong 1 , Aimin Zhou 1 , Kun Qiao 1
Affiliation  

The gene encoding a putative phosphatidate phosphatase (PAP) from tolerant saline-alkali (TSA) Chlorella, ChPAP, was identified from a yeast cDNA library constructed from TSA Chlorella after a NaCl treatment. ChPAP expressed in yeast enhanced its tolerance to NaCl and sorbitol. The ChPAP protein from a GFP-tagged construct localized to the plasma membrane and the lumen of vacuoles. The relative transcript levels of ChPAP in Chlorella cells were strongly induced by NaCl and sorbitol as assessed by northern blot analyses. Thus, ChPAP may play important roles in promoting Na-ion movement into the cell and maintaining the cytoplasmic ion balance. In addition, ChPAP may catalyze diacylglycerol pyrophosphate to phosphatidate in vacuoles.



中文翻译:

赋予 NaCl 和山梨糖醇耐受性的耐受盐碱小球藻磷脂酸磷酸酶的分子表征

编码假定的基因 磷脂酸磷酸酶 (行动计划) 来自耐盐碱 (TSA) 小球藻, 呼吸机, 从由 TSA 构建的酵母 cDNA 文库中鉴定 小球藻 NaCl 处理后。 呼吸机在酵母中表达的 增强其对 NaCl 和山梨糖醇的耐受性。来自 GFP 标记构建体的 ChPAP 蛋白定位于质膜和液泡腔。相对转录水平呼吸机小球藻通过Northern印迹分析评估,NaCl和山梨糖醇强烈诱导细胞。因此,ChPAP 可能在促进钠离子进入细胞和维持细胞质离子平衡方面发挥重要作用。此外,ChPAP 可在液泡中催化二酰基甘油焦磷酸转化为磷脂酸。

更新日期:2021-09-28
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