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Enabling High Structural Specificity to Lipidomics by Coupling Photochemical Derivatization with Tandem Mass Spectrometry
Accounts of Chemical Research ( IF 18.3 ) Pub Date : 2021-09-27 , DOI: 10.1021/acs.accounts.1c00419
Xiaoxiao Ma 1 , Wenpeng Zhang 1 , Zishuai Li 1 , Yu Xia 2 , Zheng Ouyang 1
Affiliation  

Lipids have pivotal roles in many biological processes, including energy storage, signal transduction, and plasma membrane formation. A disruption of lipid homeostasis is found to be associated with a range of diseases, such as cardiovascular diseases, diabetes, and cancer. Fundamental lipid biology and disease diagnostics can benefit from monitoring lipid changes in cells, tissues, organs, or the whole biological system. Therefore, it is important to develop lipid analysis tools to achieve comprehensive lipid characterization and quantitation. Over the past two decades, mass spectrometry (MS) has become the method of choice for qualitative and quantitative analyses of lipids, owing to its high sensitivity, multiplexed analysis, and soft ionization features. With the rapid development and adoption of ultrahigh-resolution MS, isobaric lipids can now be routinely resolved. By contrast, the structural characterization and quantitation of isomeric lipids remain an analytical challenge. Although some lipid C═C location or sn-isomers can be resolved by chromatography, ion mobility, or selective ionization approaches, a detailed structural characterization on the lipidome-wide level needs to be achieved.

中文翻译:

通过将光化学衍生化与串联质谱联用,实现脂质组学的高结构特异性

脂质在许多生物过程中起着关键作用,包括能量储存、信号转导和质膜形成。发现脂质稳态的破坏与一系列疾病有关,例如心血管疾病、糖尿病和癌症。基本的脂质生物学和疾病诊断可以受益于监测细胞、组织、器官或整个生物系统中的脂质变化。因此,开发脂质分析工具以实现全面的脂质表征和定量非常重要。在过去的 20 年里,质谱 (MS) 因其高灵敏度、多路分析和软电离特性而成为脂质定性和定量分析的首选方法。随着超高分辨率 MS 的快速发展和采用,现在可以常规分离同量异位脂。相比之下,异构脂质的结构表征和定量仍然是一个分析挑战。虽然一些脂质 C=C 位置或sn-异构体可以通过色谱、离子迁移或选择性电离方法解决,需要在脂质组范围内实现详细的结构表征。
更新日期:2021-10-19
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