The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 antibody detection with a limit of detection (LoD) down to 10^–18 M (equivalent to 10^–16 g mL^–1) level. The g-FETs are modified with spike S1 proteins, and the SARS-CoV-2 antibody biorecognition events occur in the vicinity of the graphene surface, yielding an LoD of ∼150 antibodies in 100 μL full serum, which is the lowest LoD value of antibody detection. The diagnoses time is down to 2 min for detecting clinical serum samples. As such, the g-FETs leverage rapid and precise SARS-CoV-2 screening and also hold great promise in prevention and control of other epidemic outbreaks in the future.

中文翻译：

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石墨烯场效应晶体管对 SARS-CoV-2 抗体的超灵敏检测

SARS-CoV-2的快速传播已严重威胁公众健康。建立灵敏的SARS-CoV-2检测方法对于遏制全球大流行具有重要意义。在这里，我们开发了石墨烯场效应晶体管 (g-FET) 生物传感器并实现了超灵敏的 SARS-CoV-2 抗体检测，检测限 (LoD) 低至 10 ^–18 M（相当于 10 ^–16 g mL ^–1） 等级。g-FET 用刺突 S1 蛋白修饰，SARS-CoV-2 抗体生物识别事件发生在石墨烯表面附近，在 100 μL 全血清中产生约 150 个抗体的 LoD，这是最低的 LoD 值抗体检测。检测临床血清样本的诊断时间缩短至 2 分钟。因此，g-FETs 可以利用快速和精确的 SARS-CoV-2 筛查，并且在未来其他流行病爆发的预防和控制中也有很大的希望。